C. difficile Toxin B/TcdB Antibody

Catalog # Availability Size / Price Qty
AF6246
AF6246-SP
Detection ofC. difficileToxin B/TcdB by Western Blot.
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Product Details
Citations (4)
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C. difficile Toxin B/TcdB Antibody Summary

Species Reactivity
C. difficile
Specificity
Detects C. difficile Toxin B/TcdB in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant C. difficile Toxin B/TcdB
Ser2-Leu543
Accession # P18177
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of<EM>C. difficile</EM>Toxin B/TcdB antibody by Western Blot. View Larger

Detection ofC. difficileToxin B/TcdB by Western Blot. Western blot shows recombinantC. difficileToxin B/TcdB (Catalog # 6246-GT). PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. difficileToxin B/TcdB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Toxin B/TcdB at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Toxin B/TcdB

Clostridium difficile is the leading cause of hospital-acquired diarrhea, known as C. difficile-associated disease. The estimated number of cases of C. difficile-associated disease exceeds 250,000 per year (1), with health care costs approaching US $1 billion annually (2). The major virulence factors produced by C. difficile are two toxins, TcdA and TcdB. Both toxins can monoglucosylate and inactivate Rho family small GTPases within target cells, leading to disruption of vital signaling pathways in the cell, subsequently causing diarrhea, inflammation, and damage of colonic mucosa (3, 4, 5). Both toxins have a similar tripartite structure comprised of an N‑terminal glucosyltransferase domain, a C-terminal receptor binding domain, and a small hydrophobic span possibly involved in toxin translocation (6). Our recombinant TcdB consists of the enzymatic domain. Both TcdA and TcdB also have potassium-dependent UDP-Glc hydrolase activity, which is essentially glucosyltransferase activity with water as the acceptor molecule (7). Under same conditions, UDP-glucose hydrolysis by TcdB occurs at a rate about 5-fold greater than that of TcdA.

References
  1. Wilkins, T.D. and Lyerly, D.M. (2003) J. Clin. Microbiol 41:53.
  2. Kyne, L. et al. (2002) Clin. Infect. Dis. 34:346.
  3. Voth, D.E. and Ballard, J.D. (2005) Clin. Microbiol. Rev. 18:247.
  4. Chaves-Olarte, E. et al. (1996) J. Biol. Chem. 271:6925.
  5. Just I, et al. (1995) J. Biol. Chem. 270:13932.
  6. Hammond, G.A. and Johnson, J.L. (1995) Microb. Pathog. 19:203.
  7. Ciesla, W.P. Jr. and Bobak, D.A. (1998) J. Biol. Chem. 273:16021.
Entrez Gene IDs
4914074 (C. difficile)
Alternate Names
TcdB; toxB

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Citations for C. difficile Toxin B/TcdB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Intestinal bile acids directly modulate the structure and function of C. difficile TcdB toxin
    Authors: J Tam, S Icho, E Utama, KE Orrell, RF Gómez-Biag, CM Theriot, HK Kroh, SA Rutherford, DB Lacy, RA Melnyk
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. Host-targeted niclosamide inhibits C. difficile virulence and prevents disease in mice without disrupting the gut microbiota
    Authors: J Tam, T Hamza, B Ma, K Chen, GL Beilhartz, J Ravel, H Feng, RA Melnyk
    Nat Commun, 2018;9(1):5233.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Intrinsic Toxin-Derived Peptides Destabilize and Inactivate Clostridium difficile TcdB
    Authors: JL Larabee, SJ Bland, JJ Hunt, JD Ballard
    MBio, 2017;8(3):.
    Species: Hamster
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Amino Acid Differences in the 1753-to-1851 Region of TcdB Influence Variations in TcdB1 and TcdB2 Cell Entry
    Authors: JJ Hunt, JL Larabee, JD Ballard
    mSphere, 2017;2(4):.
    Species: Chinese Hamster
    Sample Types: Cell Lysates
    Applications: Western Blot

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