c-Maf Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24551

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse

Predicted:

Monkey (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all c-Maf Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 75-110 of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|.

Reactivity Notes

Chimpanzee (87%), Bovine, Equine (81%).

Specificity

100% homologous in human isoforms CRA_a, CRA_b, and CRA_c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for c-Maf Antibody - BSA Free

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf Antibody [NBP2-24551] - SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4C, overnight. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.
Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.

Applications for c-Maf Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml

Reviewed Applications

Read 3 reviews rated 3.3 using NBP2-24551 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Maf

c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)

Alternate Names

CCA4, cMaf, MAF2

Gene Symbol

MAF

UniProt

O75444

Additional c-Maf Products

Product Documents for c-Maf Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for c-Maf Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for c-Maf Antibody - BSA Free

Customer Reviews for c-Maf Antibody - BSA Free (3)

3.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
33%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • c-Maf Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 09/13/2021
    Western Blot: SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4℃, overnight.
    c-Maf Antibody - BSA Free NBP2-24551
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: M1/M2 cell lines
    Species: Human
    Verified Customer | Posted 11/11/2015
  • Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: Multiple myeloma cells lines MY5 & RPMI-8226 and T cell leukemia cell line Jurkat cytospins
    Species: Human
    Verified Customer | Posted 07/28/2015
    IFF/ICC staining of MY5, RPMI-8226 and Jurkat cells with NBP2-24551
    c-Maf Antibody - BSA Free NBP2-24551

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Showing  1 - 3 of 3 reviews Showing All

Protocols

View specific protocols for c-Maf Antibody - BSA Free (NBP2-24551):

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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FAQs

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Associated Pathways

Th2 Differentiation Pathway Th2 Differentiation Pathway Thumbnail