c-Rel Antibody - BSA Free

Name: c-Rel Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB600-474
Price: 619 USD
Availability: InStock

Novus Biologicals | Catalog # NB600-474

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Western Blot, ELISA, Simple Western, Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Format

BSA Free

View all c-Rel Primary Antibodies »

Product Specifications

Immunogen

c-Rel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: Q04864)

Reactivity Notes

This antibody may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the c-Rel subunit.

Localization

Nuclear

Specificity

Anti-c-Rel may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the c-Rel subunit.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for c-Rel Antibody - BSA Free

Western Blot: c-Rel Antibody [NB600-474]

Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.

c-Rel Antibody

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.

Applications for c-Rel Antibody - BSA Free

Application

Recommended Usage

ELISA

1:5000-1:25000

Gel Super Shift Assays

1:500

Simple Western

1:50

Western Blot

1:500-1:3000

Application Notes

This product was tested by ELISA and by immunoblot. This product is suitable for the detection by immunoblot of human NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 ul per assay.

Simple Western Antibody Database for Simple Western validation: tested in HEK-/+UV, whole cell lysate; separated by charge, antibody dilution of 1:50.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: c-Rel

c Rel is a protooncogene encoding a subunit of the NF kappa B family of transcription factors. In B and T cells, it is involved in the regulation of genes controlling cell division and immune function.

Long Name

Cellular Repressor of E1A-stimulated Genes

Alternate Names

cRel, REL

Entrez Gene IDs

5966 (Human); 19696 (Mouse)

Gene Symbol

REL

UniProt

Q04864 (Human)

Additional c-Rel Products

Product Documents for c-Rel Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for c-Rel Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.