C5b-9 Antibody (aE11) [FITC]

Novus Biologicals | Catalog # NBP1-05120F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Baboon, Bacteria, Equine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot (Negative)

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # aE11
View all C5b-9 Primary Antibodies »

Product Specifications

Immunogen

Purified C5b-9

Epitope

Binds to a neoepitope exposed on C9.

Reactivity Notes

Use in Bacteria reported in scientific literature (PMID:32900809).

Specificity

Binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for C5b-9 Antibody (aE11) [FITC]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot (Negative)

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: C5b-9

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Alternate Names

MAC, Membrane attack complex, sC5-b9, sC5b-9, TCC, Terminal complement complex

Additional C5b-9 Products

Product Documents for C5b-9 Antibody (aE11) [FITC]

Certificate of Analysis

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Product Specific Notices for C5b-9 Antibody (aE11) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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