CACNA2D1 Antibody (20A)

Novus Biologicals | Catalog # NB120-2864

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Guinea Pig, Rabbit

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 20A
View all CACNA2D1 Primary Antibodies »

Product Specifications

Immunogen

Purified rabbit dihydropyridine receptor.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 16938495). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from skeletal and cardiac muscle. Detects a band at 220 kDa under non-reducing and a 143 kDa band under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with NB120-2864 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CACNA2D1 Antibody (20A)

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Primary antibody dilution- 1:1000 for 2 Hours at RT, in blocking buffer, 5% Milk in TBS-0.05% Tween : Blocking Overnight at +4 degree C. Secondary antibody : 1:2000 in blocking buffer, 1 hour at RT. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized mouse skeletal muscle tissue.
Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of C6 cells compared to an isotype control (blue).
Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates.
Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized human skeletal muscle tissue.
Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of SH-SY5Y cells compared to an isotype control (blue).
Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864]

Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of Neuro-2a cells compared to an isotype control (blue).

Applications for CACNA2D1 Antibody (20A)

Application
Recommended Usage

Flow Cytometry

1:100 - 1:200

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 8070639).

Reviewed Applications

Read 1 review rated 5 using NB120-2864 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CACNA2D1

FUNCTION: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.; Subcellular location: Membrane; Single-pass type I membrane protein; Tissue specificity: Central nervous system, skeletal muscle and aorta tissues.; Miscellaneous: Binds gabapentin, an antiepileptic drug.

Long Name

Voltage-dependent calcium channel subunit alpha-2/delta-1

Alternate Names

CACNL2A, CCHL2A, MHS3

Entrez Gene IDs

781 (Human); 12293 (Mouse); 25399 (Rat); 100009105 (Rabbit)

Gene Symbol

CACNA2D1

UniProt

P13806 (Rabbit)

Additional CACNA2D1 Products

Product Documents for CACNA2D1 Antibody (20A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CACNA2D1 Antibody (20A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CACNA2D1 Antibody (20A)

Customer Reviews for CACNA2D1 Antibody (20A) (1)

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Customer Images

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  • CACNA2D1 Antibody (20A)
    Name: Anmol Kumar
    Application: Western Blot
    Sample Tested: ARPE19 cell lysate
    Species: Human
    Verified Customer | Posted 06/06/2018
    ARPE19 cell lysate
    CACNA2D1 Antibody (20A) NB120-2864

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Protocols

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FAQs for CACNA2D1 Antibody (20A)

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  • Q: We bought a vial of CACNA2D1 Antibody (20A) (NB120-2864) from you. But the datasheet doesn't tell us the antibody's concentration and positive control tissues. Can you supply the information for me? I wonder if the antibody has a total concentration or concentration range. You know if we don't have the concentration information, we can't ascertain the IgG's concentration for negative control.

    A: This product is unpurified (diluted ascites) and therefore, concentration is not relevant. I talked to the lab team regarding your concerns and they told me that the total protein concentration in the ascites was around 2.2 mg/mL. Generally, 90% of the ascites is IgG, therefore, I assume that the IgG concentration in your vial should be around 1.98 mg/ml. You can use our CACNA2D1 overexpression lysate (# NBP2-08998) or skeletal/cardiac muscle tissue membrane preparations as positive control for Western Blot assay. As far as IHC application is concerned, you may use skeletal/cardiac muscle tissue sections as positive control wherein this product will show selective cytoplasmic expression of CACNA2D1.

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