Calbindin D-28K Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-50028

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
View all Calbindin D-28K Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Calbindin D-28K. [UniProt# P05937]

Reactivity Notes

Mouse reported from a verifed customer review.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calbindin D-28K Antibody - BSA Free

Western Blot: Calbindin D-28K Antibody [NBP2-50028]

Western Blot: Calbindin D-28K Antibody [NBP2-50028]

Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028]

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028]

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.
Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028]

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028]

Calbindin-D-28K-Antibody-Immunohistochemistry-NBP2-50028-img0007.jpg
Western Blot: Calbindin D-28K Antibody [NBP2-50028]

Western Blot: Calbindin D-28K Antibody [NBP2-50028]

Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin.
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028]

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028]

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Adult rat brain cortex (Left) and striatum (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with NBP2-50028 (1:1,000, red), and monoclonal mouse anti-RBFOX3/NeuN (NBP1-92693; green). Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express RBFOX3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.
Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat cerebellum section stained with chicken Calbindin D-28K pAb, dilution 1:2,000 (Green), and costained with rabbit MeCP2 pAb, dilution 1:5,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. Calbindin D-28K, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.
Calbindin D-28K Antibody

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] -

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - C1q is expressed in retina microglia during development. (A) qRT-PCR for C1q at P9, P14, & 14 weeks. Values represent the fold mRNA expression level relative to the levels detected in adult animals following normalization to GAPDH. (B) Representative fluorescent in situ hybridization images at P13 of C1q (green) co-stained with either Iba1 (magenta) or calbindin (red) to label microglia & horizontal cells, respectively. C1q colocalizes with microglia but not with horizontal cells in the outer retina at this time (arrowheads). Inset shows C1q expression in Iba1-positive microglia that is absent in calbindin-positive horizontal cells. Scale bars = 25 & 10 μm (inset). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33177995), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Calbindin D-28K Antibody

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] -

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Myh14 localizes to apical membranes of Atp6v1b1‐positive intercalated cells in the distal tubule. Adult kidney sections were stained for calbindin‐D28K, Atp6v1b1, Aqp2, & Myh14 to identify intercalated cells within the distal & connecting tubule segments as well as to confirm Myh14 localization to the intercalated cell type. Cells with positive staining for Atp6v1b1 (red) can be found in tubule segments positive for calbindin‐D28K only (A–D) as well as in tubules positive for both calbindin‐D28K & Aqp2 (E–H); in both cases Atp6v1b1 staining is observed along the basolateral membrane indicating beta ‐ intercalated cell type (arrowhead, D&H). We also observed Atp6v1b1 staining in majority of the tubules along the apical membrane indicating the alpha ‐ intercalated cell type (arrowhead, I–L). Myh14 localized to the apical membrane of the intercalated cell type in the distal tubule (arrowhead, M–P). Sections were mounted with vectashield containing DAPI to stain the nucleus (blue; D, H, L & P). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29208685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Calbindin D-28K Antibody

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] -

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - Horizontal cells develop normally with the loss of either C3aR or CR3. (A,B) qRT-PCR for complement proteins C3aR & CR3 at P6, P13, & adult in wild type mice. Values represent the fold mRNA expression level relative to the levels detected in control animals following normalization to GAPDH. N = 3 animals. Representative images of horizontal cells (C, anti-calbindin, cyan) & quantifications (D,E) of ectopic horizontal cell neurites in control & C3aR–/– or CR3–/– mice at P13. Horizontal cell neurites in C3aR–/– & CR3–/– mice remain confined to the OPL, showing no increase in the number (D) or length (E) of ectopic neurites (n = 52 sprouts from four control mice; n = 84 sprouts from four C3aR–/– mice; n = 67 sprouts from four CR3–/– mice). Scale bars = 25 μm. Data are represented as the mean ± SEM (D) or as beeswarm SuperPlots (E) in which individual horizontal cell sprout values are presented together with the mean from each animal ± the SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33177995), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Calbindin D-28K Antibody

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] -

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Myh10 & Myh14 are the NM2 isoforms expressed in the distal & connecting renal tubules. Adult kidney sections were stained for calbindin‐D28K, Aqp2 & one of the NM2 isoforms. Myh9 (A–D) was not expressed in tubules positive for calbindin‐D28K & Aqp2; although adjacent tubules were positive for Myh9 (arrow). Myh10 (E–H) was expressed at low levels at the cell–cell junctions in calbindin‐D28K only positive tubules (white arrowhead), & in tubules positive for both calbindin‐D28K & Aqp2, Myh10 localized to cell–cell junctions, apical, & basolateral membrane (F & H, yellow arrowhead). Myh14 (I–L) was expressed on the apical membrane of calbindin‐D28K & Aqp2 null cells (arrowheads) in the distal tubular segments. Sections were mounted with vectashield containing DAPI to stain the nucleus (blue; D, H, & L). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29208685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Calbindin D-28K Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:1000 - 1:5000
Application Notes
In Western blot, this antibody recognizes a clear band at ~28 kDa in rat brain lysates which represents Calbindin and it reacts only with Calbindin protein, and not other calcium-binding proteins. A band maybe seen at ~60 kDa and is most likely the dimer of Calbindin. ICC/IF reported from a verified customer review. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 29208685).

Reviewed Applications

Read 1 review rated 5 using NBP2-50028 in the following applications:

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

UniProt

P05937

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Calbindin D-28K Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calbindin D-28K Antibody - BSA Free

Customer Reviews for Calbindin D-28K Antibody - BSA Free (1)

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Customer Images

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  • Calbindin D-28K Antibody
    Name: Julia Tarnick
    Application: Immunocytochemistry
    Sample Tested: Embryonic kidney
    Species: Mouse
    Verified Customer | Posted 08/04/2018
    cultured embryonic kidney, stained with Calbindin and Dapi
    Fixation: 4% PFA, 30min RT Permeabilization: 100 % methanol -20°C, 20 min blocking buffer: 3% donkey serum antibody dilution: 1:200 in blocking buffer, incubation overnight 4°C
    Calbindin D-28K Antibody - BSA Free NBP2-50028

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FAQs for Calbindin D-28K Antibody - BSA Free

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  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

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