Calcineurin B Antibody (4E1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005534-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E1

Format

Azide and BSA Free
View all Calcineurin B Primary Antibodies »

Product Specifications

Immunogen

PPP3R1 (AAH27913, 1 a.a. ~ 170 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Specificity

PPP3R1 - protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Calcineurin B Antibody (4E1) - Azide and BSA Free

Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01]

Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01]

Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01] - PPP3R1 monoclonal antibody (M01), clone 4E1. Analysis of PPP3R1 expression in rat brain.
Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01]

Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01]

Immunocytochemistry/Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01] - Analysis of monoclonal antibody to PPP3R1 on HeLa cell. Antibody concentration 25 ug/ml.
ELISA: Calcineurin B Antibody (4E1) [H00005534-M01]

ELISA: Calcineurin B Antibody (4E1) [H00005534-M01]

ELISA: Calcineurin B Antibody (4E1) [H00005534-M01] - Calcineurin B Antibody (4E1) [H00005534-M01] - Detection limit for recombinant GST tagged PPP3R1 is approximately 0.1ng/ml as a capture antibody.

Applications for Calcineurin B Antibody (4E1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calcineurin B

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity

Alternate Names

CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1

Entrez Gene IDs

5534 (Human)

Gene Symbol

PPP3R1

OMIM

601302 (Human)

UniProt

AAH27913 (Human)

Additional Calcineurin B Products

Product Documents for Calcineurin B Antibody (4E1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calcineurin B Antibody (4E1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calcineurin B Antibody (4E1) - Azide and BSA Free

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Protocols

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FAQs

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