Calcineurin B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15664

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calcineurin B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcineurin B. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calcineurin B Antibody - BSA Free

Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody [NBP2-15664]

Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody [NBP2-15664]

Immunocytochemistry/Immunofluorescence: Calcineurin B Antibody [NBP2-15664] - Calcineurin B antibody detects Calcineurin B protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin B stained by Calcineurin B antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Calcineurin B Antibody [NBP2-15664]

Immunohistochemistry-Paraffin: Calcineurin B Antibody [NBP2-15664]

Immunohistochemistry-Paraffin: Calcineurin B Antibody [NBP2-15664] - Analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664]

Western Blot: Calcineurin B Antibody [NBP2-15664] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:50000.
Calcineurin B Antibody

Western Blot: Calcineurin B Antibody [NBP2-15664] -

Western Blot: Calcineurin B Antibody [NBP2-15664] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP2-15664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Calcineurin B Antibody

Western Blot: Calcineurin B Antibody [NBP2-15664] -

Western Blot: Calcineurin B Antibody [NBP2-15664] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP2-15664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Calcineurin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:50000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calcineurin B

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Alternate Names

CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1

Gene Symbol

PPP3R1

UniProt

P63098

Additional Calcineurin B Products

Product Documents for Calcineurin B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Calcineurin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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