Calcium-sensing R/CaSR Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38622

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calcium-sensing R/CaSR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLI

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32824949).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calcium-sensing R/CaSR Antibody - BSA Free

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).
Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Calcium-sensing R/CaSR Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:32824949)

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calcium-sensing R/CaSR

Calcium-Sensing Receptor (CaSR) is a member of the G protein-coupled receptor family. CaSR is an integral membrane protein that senses changes in the extracellular concentration of calcium ions and controls the release of parathyroid hormone. The activity of the calcium-sensing receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.

Mutations that inactivate CaSR lead to familial hypocalciuric hypercalcemia, whereas mutations that activate CaSR cause autosomal dominant hypocalcemia. Calcium-Sensing Receptor antibodies are useful tools for parathyroid studies and research on cellular calcium homeostasis regulation.

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Gene Symbol

CASR

UniProt

P41180 (Rat)

Additional Calcium-sensing R/CaSR Products

Product Documents for Calcium-sensing R/CaSR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Calcium-sensing R/CaSR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calcium-sensing R/CaSR Antibody - BSA Free

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Protocols

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