Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria, Bovine, Chicken

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D1

Format

BSA Free
View all Calmodulin 1 Primary Antibodies »

Product Specifications

Immunogen

Calmodulin purified from Dictyostelium discoideum.

Reactivity Notes

E. coli. Chlamydomonas reinhardtii and Dictyostelium discoideum. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Calmodulin Antibody (2D1) - BSA Free (NB120-2860) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Calmodulin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Calmodulin Antibody (2D1) - BSA Free

Western Blot: Calmodulin Antibody (2D1)BSA Free [NB120-2860]

Western Blot: Calmodulin Antibody (2D1)BSA Free [NB120-2860]

Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.
Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).
Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).

Applications for Calmodulin Antibody (2D1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

2 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:20

Immunohistochemistry-Paraffin

1:20

Western Blot

1:500
Application Notes
WB: Detects an approx. 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EDTA to buffers completely inhibits antibody staining. IHC: Staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calmodulin 1

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Alternate Names

CALM1, CALML2, CaM, CAM1, CAMI, CPVT4, DD132

Entrez Gene IDs

24242 (Rat)

Gene Symbol

CALM1

Additional Calmodulin 1 Products

Product Documents for Calmodulin Antibody (2D1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Calmodulin Antibody (2D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calmodulin Antibody (2D1) - BSA Free

Customer Reviews for Calmodulin Antibody (2D1) - BSA Free

There are currently no reviews for this product. Be the first to review Calmodulin Antibody (2D1) - BSA Free and earn rewards!

Have you used Calmodulin Antibody (2D1) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Calmodulin Antibody (2D1) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

    • Q: I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K
      A: Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.

    • A: Indeed, it can recognize both the native and denatured calmodulin.
      We have tested applications for both conformations:
      Native conformations being available in ICC/FLow/IHC and ELISA applications, while the denatured application being western blot applications.
Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies