Calponin 1 Antibody (rCNN1/6918)

Novus Biologicals | Catalog # NBP3-49074

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rCNN1/6918 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fragment (around aa197-297) of human CNN1 protein (exact sequence is proprietary) (Uniprot: P51911)

Localization

Cytoplasm

Specificity

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for Calponin 1 Antibody (rCNN1/6918)

Calponin 1 Antibody (rCNN1/6918)

Immunohistochemistry-Paraffin: Calponin 1 Antibody (rCNN1/6918) [NBP3-49074] -

Formalin-fixed, paraffin-embedded human uterus stained with Calponin 1 Antibody (rCNN1/6918). HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.
Calponin 1 Antibody (rCNN1/6918)

Western Blot: Calponin 1 Antibody (rCNN1/6918) [NBP3-49074] -

Western Blot Analysis of Colon lysate using Calponin 1 Antibody (rCNN1/6918).

Applications for Calponin 1 Antibody (rCNN1/6918)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (rCNN1/6918)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Calponin 1 Antibody (rCNN1/6918)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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