Calretinin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88220

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calretinin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSAEFMEAWRKY

Marker

Mesothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calretinin Antibody - BSA Free

Calretinin Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Calretinin Antibody - BSA Free [NBP1-88220]

Immunocytochemistry/ Immunofluorescence: Calretinin Antibody - BSA Free [NBP1-88220]

Staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum, skeletal muscle, stomach and testis using Anti-CALB2 antibody NBP1-88220 (A) shows similar protein distribution across tissues to independent antibody NBP1-88221 (B).
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining in human testis and skeletal muscle tissues using NBP1-88220 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220]

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.
Calretinin Antibody - BSA Free Western Blot: Calretinin Antibody - BSA Free [NBP1-88220]

Western Blot: Calretinin Antibody - BSA Free [NBP1-88220]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Calretinin Antibody - BSA Free Western Blot: Calretinin Antibody - BSA Free [NBP1-88220]

Western Blot: Calretinin Antibody - BSA Free [NBP1-88220]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CALB2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for Calretinin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-88220 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Calretinin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calretinin Antibody - BSA Free

Customer Reviews for Calretinin Antibody - BSA Free (1)

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  • Calretinin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Brain tissue
    Species: Rat
    Verified Customer | Posted 01/07/2019
    Rat brain tissue - Alcohol fixed-paraffin embedded
    Calretinin Antibody - BSA Free NBP1-88220

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Protocols

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FAQs

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