CaM Kinase II alpha Antibody (JM11-07)

Novus Biologicals | Catalog # NBP2-67207

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM11-07 expressed in HEK293
View all CaM Kinase II alpha Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CaM Kinase II alpha aa 37-71 / 478. (SwissProt: Q9UQM7 Human; SwissProt: P11798 Mouse; SwissProt: P11275 Rat)

Localization

Cell junction, Cell projection, Synapse.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II alpha Antibody (JM11-07)

Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Flow cytometric analysis of CaM Kinase II alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaM Kinase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for CaM Kinase II alpha Antibody (JM11-07)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:50

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Gene Symbol

CAMK2A

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody (JM11-07)

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Product Specific Notices for CaM Kinase II alpha Antibody (JM11-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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