CaMKII beta Antibody - BSA Free

Novus Biologicals | Catalog # NB120-22131

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CaMKII beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 499-513 (VRRGSGTPEAEAPRQ) of human CAMKII beta.

Localization

Cytoplasmic, widely expressed; expressed in adult and fetal brain. (Expression is lightly lower in fetal brain.)

Specificity

Human CAMKII beta GenBank no. GENE ID: 816 CAMK2B is a 664 amino acid protein. However, several isoforms of varying amino acid sizes have been described for CAMKII beta. Researchers are encouraged to utilize the sequence information used for immunogen (VRRGSGTPEAEAPRQ) to gain additional information about these isoforms and various molecular weights which may be detected by western blot analysis.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaMKII beta Antibody - BSA Free

Western Blot: CaMKII beta Antibody [NB120-22131]

Western Blot: CaMKII beta Antibody [NB120-22131]

Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NB120-22131]

Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NB120-22131]

Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [NB120-22131] - Staining of CAMKIIb using NB120-22131 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.
Western Blot: CaMKII beta Antibody [NB120-22131]

Western Blot: CaMKII beta Antibody [NB120-22131]

Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.

Applications for CaMKII beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 20708685)

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera with 50% Glycerol

Format

BSA Free

Preservative

0.025% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaMKII beta

The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.

Long Name

Calcium/calmodulin-dependent protein kinase type II subunit beta

Alternate Names

CAM2, CAMKB, EC 2.7.11.17

Entrez Gene IDs

816 (Human); 12323 (Mouse); 24245 (Rat)

Gene Symbol

CAMK2B

Additional CaMKII beta Products

Product Documents for CaMKII beta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CaMKII beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CaMKII beta Antibody - BSA Free

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Protocols

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FAQs

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