CAMP/Cathelicidin Antibody (OSX12)

Novus Biologicals | Catalog # NBP1-46781

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OSX12
View all CAMP/Cathelicidin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human CAMP/Cathelicidin conjugated to an immunogenic carrier protein has been used as the antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CAMP/Cathelicidin Antibody (OSX12)

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer).
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells.
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/ Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781]

Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells

Applications for CAMP/Cathelicidin Antibody (OSX12)

Application
Recommended Usage

Flow Cytometry

5-10 ug/ml

Immunocytochemistry/ Immunofluorescence

5-10 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

5-10 ug/ml
Application Notes
The optimal concentration should be determined by the end user. Use in immoprecipitation reported in scientific literature (PMID 26508735)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Sterilized water

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CAMP/Cathelicidin

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Alternate Names

18 kDa cationic antimicrobial protein, Antibacterial peptide FALL-39, CAP18, CAP-18, cathelicidin antimicrobial peptide, CRAMP, epididymis secretory sperm binding protein, FALL39, FALL-39, FALL-39 peptide antibiotic, hCAP-181, HSD26, LL37

Entrez Gene IDs

820 (Human)

Gene Symbol

CAMP

UniProt

P49913 (Human)

Additional CAMP/Cathelicidin Products

Product Documents for CAMP/Cathelicidin Antibody (OSX12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CAMP/Cathelicidin Antibody (OSX12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CAMP/Cathelicidin Antibody (OSX12)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CAMP/Cathelicidin Antibody (OSX12)

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  • Q: I would like to get a technical information about your antibody Cathelicidin Antibody (OSX12), product-number NBP1-46781. Does this antibody recognize specifically the C-terminal antimicrobial domain LL-37, which is liberated by proteinase 3 from cathelicidin?

    A: A synthetic peptide from human Cathelicidin antimicrobial peptide (LL-37) conjugated to an immunogenic carrier protein was used as the immunogen to generate our Cathelicidin antibody with catalogue number NBP1-46781. The peptide immunogen was derived from the C-terminus of the protein, however the exact length and amino acid sequence is proprietary and I am, unfortunately, unable to disclose it.

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