Canine CD4 PE‑conjugated Antibody

Name: Canine CD4 PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB2410P
Price: 369 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # FAB2410P

(1)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Canine

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG_2B Clone # 296712

View all CD4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine CD4
Val25-Lys401
Accession # P33705

Specificity

Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD4, recombinant mouse CD4, and recombinant feline CD4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Canine CD4 PE‑conjugated Antibody

Detection of CD4 in Lymphocytes in Canine PBMCs by Flow Cytometry.

Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB2410P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Canine CD4 PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Lymphocytes in canine peripheral blood mononuclear cells

Reviewed Applications

Read 1 review rated 4 using FAB2410P in the following applications:

Flow Cytometry (1 Review)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD4

CD4 is a 56-58 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on a variety of cell types including T helper cells, follicular helper cells, neurons, T regulatory cells, microglia, and monocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. Over aa 25-401, canine CD4 shares 56% amino acid identity with human CD4.