Canine IL‑21 Antibody

Name: Canine IL‑21 Antibody
Brand: R&D Systems
SKU: AF5849
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # AF5849

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Product Specifications
Scientific Data
Applications
Preparation & Storage
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Background
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Key Product Details

Species Reactivity

Canine

Applications

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all IL-21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IL-21
His18-Ser146
Accession # NP_001003347

Specificity

Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Canine IL‑21 Antibody

Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody.

Recombinant Canine IL-21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND₅₀ is typically 2-10 µg/mL.

Applications for Canine IL‑21 Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. Parrish-Novak, J. et al. (2000) Nature 408:57. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Canine IL‑21.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21

Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4⁺ T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1‑5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL‑21 R, a member of the class I cytokine receptor family (1‑2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1‑2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4⁺ T cells and B cells, the functional activity of CD8⁺ T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5‑6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5‑6). IL‑21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori‑related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL‑21 is also involved in controlling chronic viral infections (7).