Canine IL-21 Antibody

Catalog # Availability Size / Price Qty
AF5849
AF5849-SP
Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody.
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Canine IL-21 Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant canine IL-21
His18-Ser146
Accession # NP_001003347
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. Parrish-Novak, J. et al. (2000) Nature 408:57. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Canine IL‑21.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. View Larger

Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. Recombinant Canine IL‑21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21

Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4+ T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1‑5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL‑21 R, a member of the class I cytokine receptor family (1‑2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1‑2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4+ T cells and B cells, the functional activity of CD8+ T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5‑6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5‑6). IL‑21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori‑related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL‑21 is also involved in controlling chronic viral infections (7).

References
  1. Monteleone, G. et al. (2009) Cytokine Growth Factor Rev. 20:185.
  2. Parrish-Novak, J. et al. (2000) Nature 408:57.
  3. Coquet, J.M. et al. (2007) J. Immunol.178:2827.
  4. Vogelzang, A. et al. (2008) Immunity 29:127.
  5. Monteleone, G. et al. (2008) Trends Immunol. 29:290.
  6. Spolski, R. et al. (2008) Curr. Opin. Immunol. 20:295.
  7. Yi, J.S. et al. (2009) Science 324:1572.
Long Name
Interleukin 21
Entrez Gene IDs
59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
Alternate Names
IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform

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