Canine IL-21 Antibody

  
  • Species Reactivity
    Canine
  • Specificity
    Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant canine IL-21
    His18-Ser146
    Accession # NP_001003347
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. Parrish-Novak, J. et al. (2000) Nature 408:57. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Canine IL‑21.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. Recombinant Canine IL‑21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21
Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4+ T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1‑5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL‑21 R, a member of the class I cytokine receptor family (1‑2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1‑2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4+ T cells and B cells, the functional activity of CD8+ T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5‑6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5‑6). IL‑21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori‑related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL‑21 is also involved in controlling chronic viral infections (7).
  • References:
    1. Monteleone, G. et al. (2009) Cytokine Growth Factor Rev. 20:185.
    2. Parrish-Novak, J. et al. (2000) Nature 408:57.
    3. Coquet, J.M. et al. (2007) J. Immunol.178:2827.
    4. Vogelzang, A. et al. (2008) Immunity 29:127.
    5. Monteleone, G. et al. (2008) Trends Immunol. 29:290.
    6. Spolski, R. et al. (2008) Curr. Opin. Immunol. 20:295.
    7. Yi, J.S. et al. (2009) Science 324:1572.
  • Long Name:
    Interleukin 21
  • Entrez Gene IDs:
    59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
  • Alternate Names:
    IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform
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