Canine VEGF Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
CAVE00
Canine VEGF ELISA Cell Culture Supernate Standard Curve
3 Images
Product Details
Procedure
Citations (6)
FAQs
Supplemental Products
Reviews

Canine VEGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
19.5 pg/mL
Assay Range
19.5 - 1,250 pg/mL (Cell Culture Supernates), 39.1 - 2,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 29.1 123 531 32.8 128 495
Standard Deviation 1.9 5 18.4 2.8 6.4 33
CV% 6.5 4.1 3.5 8.5 5 6.7

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 53.7 235 910 64.5 250 1003
Standard Deviation 3.6 10.6 46.2 5.7 17.4 61.7
CV% 6.7 4.5 5.1 8.8 7 6.2

Recovery

The recovery of canine VEGF spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 94-110
EDTA Plasma (n=4) 98 92-107
Heparin Plasma (n=4) 107 96-118
Serum (n=4) 103 87-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine VEGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Canine VEGF ELISA Linearity

Data Examples

Canine VEGF ELISA Cell Culture Supernate Standard Curve

Canine VEGF ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name:
Vascular Endothelial Growth Factor
Entrez Gene IDs:
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names:
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Cell Culture Supernate Samples or 100 µL Assay Diluent for Serum/Plasma Samples
  4.   For Cell Culture Supernate Samples:  Add 50 µL of Assay Diluent to each well.
    For Serum & Plasma Samples:  Add 100 µL of Assay Diluent to each well.

  5. 200 µL Standard or Sample for Cell Culture Supernate Samples or 100 µL Standard or Sample for Serum/Plasma Samples
  6.   For Cell Culture Supernate Samples:  Add 200 µL of Standard or sample to each well.
    For Serum & Plasma Samples:  Add 100 µL of Standard or sample to each well.
  7.   Cover with a plate sealer, and incubate at room temperature for 2 hours.
  8.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  9. 200 µL Conjugate
  10.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
  14. For Cell Culture Supernate Samples:  Incubate at room temperature for 20 minutes.
    For Serum & Plasma Samples:  Incubate at room temperature for 25 minutes.

  15. 50 µL Stop Solution
  16. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Canine VEGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. In-vitro binding analysis of anti-human vascular endothelial growth factor antibodies bevacizumab and aflibercept with canine, feline, and equine vascular endothelial growth factor
    Authors: LM Muellerlei, B Buxbaum, B Nell, DA Fux
    Res. Vet. Sci., 2019;124(0):233-238.
    Species: Canine
    Sample Types: Recombinant Protein
  2. Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs
    Authors: SH Lee, JC Ra, HJ Oh, MJ Kim, EMN Setyawan, YB Choi, JW Yang, SK Kang, SH Han, GA Kim, BC Lee
    Cell Transplant, 2019;0(0):9636897188216.
    Species: Human
    Sample Types: Serum
  3. Outcomes of Spatially Fractionated Radiotherapy (GRID) for Bulky Soft Tissue Sarcomas in a Large Animal Model
    Authors: MW Nolan, TL Gieger, AA Karakashia, MN Nikolova-K, LP Posner, DM Roback, JN Rivera, S Chang
    Technol. Cancer Res. Treat, 2017;0(0):1533034617690.
    Species: Canine
    Sample Types: Serum
  4. Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165
    Authors: HL Deissler, GK Lang, GE Lang
    Cell. Physiol. Biochem., 2017;42(4):1493-1513.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  5. Plasma N-Terminal Probrain Natriuretic Peptide, Vascular Endothelial Growth Factor, and Cardiac Troponin I as Novel Biomarkers of Hypertensive Disease and Target Organ Damage in Cats
    Authors: ES Bijsmans, RE Jepson, C Wheeler, HM Syme, J Elliott
    J. Vet. Intern. Med., 2017;0(0):.
    Species: Feline
    Sample Types: Plasma
  6. Pilot study to determine the feasibility of radiation therapy for dogs with right atrial masses and hemorrhagic pericardial effusion
    Authors: MW Nolan, MM Arkans, D LaVine, T DeFrancesc, JA Myers, EH Griffith, LP Posner, BW Keene, SP Tou, TL Gieger
    J Vet Cardiol, 2017;0(0):.
    Species: Canine
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Supplemental ELISA Products

Reviews for Canine VEGF Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Canine VEGF Quantikine ELISA Kit and earn rewards!

Have you used Canine VEGF Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review