VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.
Canine VEGF Quantikine ELISA Kit
R&D Systems | Catalog # CAVE00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
9.8 pg/mL (Cell Culture Supernates)
19.5 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
19.5 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Assay Range
19.5-1250 pg/mL (Cell Culture Supernates)
39.1-2500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
39.1-2500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Canine VEGF Quantikine ELISA Kit
The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Canine
Specificity
Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 29.1 | 123 | 531 | 32.8 | 128 | 495 |
| Standard Deviation | 1.9 | 5.0 | 18.4 | 2.8 | 6.4 | 33.0 |
| CV% | 6.5 | 4.1 | 3.5 | 8.5 | 5.0 | 6.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 53.7 | 235 | 910 | 64.5 | 250 | 1003 |
| Standard Deviation | 3.6 | 10.6 | 46.2 | 5.7 | 17.4 | 61.7 |
| CV% | 6.7 | 4.5 | 5.1 | 8.8 | 7.0 | 6.2 |
Recovery for Canine VEGF Quantikine ELISA Kit
The recovery of canine VEGF spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 94-110 |
| EDTA Plasma (n=4) | 98 | 92-107 |
| Heparin Plasma (n=4) | 107 | 96-118 |
| Serum (n=4) | 103 | 87-113 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine VEGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Canine VEGF Quantikine ELISA Kit
Canine VEGF ELISA Cell Culture Supernate Standard Curve
Canine VEGF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGF
Long Name
Vascular Endothelial Growth Factor
Alternate Names
MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF
Entrez Gene IDs
Gene Symbol
VEGFA
Additional VEGF Products
Product Documents for Canine VEGF Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Canine VEGF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Canine VEGF Quantikine ELISA Kit
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Protocols
View specific protocols for Canine VEGF Quantikine ELISA Kit (CAVE00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Cell Culture Supernate Samples: Add 50 µL of Assay Diluent to each well.
For Serum & Plasma Samples: Add 100 µL of Assay Diluent to each well. - For Cell Culture Supernate Samples: Add 200 µL of Standard or sample to each well.
For Serum & Plasma Samples: Add 100 µL of Standard or sample to each well. - Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
- For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes.
For Serum & Plasma Samples: Incubate at room temperature for 25 minutes. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent for Cell Culture Supernate Samples or 100 µL Assay Diluent for Serum/Plasma Samples
200 µL Standard or Sample for Cell Culture Supernate Samples or 100 µL Standard or Sample for Serum/Plasma Samples
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
Neural Stem Cell Differentiation Pathways & Lineage-specific Markers
VEGF - VEGF R2 Signaling Pathways
