Cannabinoid R1/CB1/CNR1 Antibody

Novus Biologicals | Catalog # NB100-2427

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Predicted:

Canine (100%), Human (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cannabinoid R1/CB1/CNR1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HTSEDGKVQVTRPDQ corresponding to internal region according to NP_001153732.1, NP_001153731.1, NP_001153730.1, NP_001153698.1, NP_149421.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24291518).

Specificity

This antibody is expected to recognize both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cannabinoid R1/CB1/CNR1 Antibody

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427]

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427]

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427] - Western blot analysis of HeLa lysate non-glycosylated (A) + peptide (B) with antibody at 1 ug/mL and HeLa lysate glycosylated (C) + peptide (D) with antibody at 0.5 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.

Applications for Cannabinoid R1/CB1/CNR1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/mL
Application Notes
Western blot: Approx 55 kDa (non-glycosylated) and 65 kDa (glycosylated) bands observed in lysates of cell line HeLa (calculated MW of 52.9 kDa according to NP_057167.2;). Both bands were successfully blocked by incubation with the immunizing peptide,

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cannabinoid R1/CB1/CNR1

Gene ID(s): 1268 (human)

Long Name

Cannabinoid Receptor 1

Alternate Names

CANN6, CannabinoidR1, CB-R, CB1, CB1A, CB1K5, CB1R, CNR1, SKR6R

Entrez Gene IDs

1268 (Human); 12801 (Mouse); 25248 (Rat)

Gene Symbol

CNR1

UniProt

NP_057167.2 (Human)

Additional Cannabinoid R1/CB1/CNR1 Products

Product Documents for Cannabinoid R1/CB1/CNR1 Antibody

Certificate of Analysis

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Product Specific Notices for Cannabinoid R1/CB1/CNR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cannabinoid R1/CB1/CNR1 Antibody

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