CapG Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15259

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CapG Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CapG. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus; Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CapG Antibody - BSA Free (NBP2-15259) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CapG Antibody - BSA Free

Immunohistochemistry-Paraffin: CapG Antibody [NBP2-15259]

Immunohistochemistry-Paraffin: CapG Antibody [NBP2-15259]

Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: CapG Antibody [NBP2-15259]

Western Blot: CapG Antibody [NBP2-15259]

Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:2000.
CapG Antibody

Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] -

Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] - CapG antibody [N3C3] detects CapG protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CapG protein stained by CapG antibody [N3C3] (NBP2-15259) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
CapG Antibody

Western Blot: CapG Antibody [NBP2-15259] -

Western Blot: CapG Antibody [NBP2-15259] - Wild-type (WT) and CAPG knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CAPG antibody [N3C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CapG Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CapG

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Long Name

Capping Protein, Gelsolin-like

Alternate Names

AFCP

Gene Symbol

CAPG

UniProt

P40121 (Human)

Additional CapG Products

Product Documents for CapG Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CapG Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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