Carboxylesterase 1 hydrolyzes ester- and amide bonds of substrates ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. It plays a major role in the pharmacokinetic behavior of many therapeutic agents containing an ester. Through de-esterification, it participates in the detoxification of drugs such as cocaine and heroin in serum and liver.
Carboxylesterase 1/CES1 Antibody
Novus Biologicals | Catalog # NBP1-00167
![Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]](https://resources.rndsystems.com/images/products/CES1-Antibody-Western-Blot-NBP1-00167-img0005.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-NTQAAQKLKDKE corresponding to internal region (near C-Terminus) according to NP_001020366.1, NP_001020365.1, NP_001257.4.
Specificity
This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Carboxylesterase 1/CES1 Antibody
Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]
Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]](https://resources.rndsystems.com/images/products/CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-00167-img0004.jpg "Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]")
Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]
Immunohistochemistry-Paraffin: CES1 Antibody [NBP1-00167] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Carboxylesterase 1/CES1 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 3 ug/ml
Immunohistochemistry-Paraffin
2 - 3 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 55-60 kDa band observed in human liver and Ileum lysates (calculated MW of 62.6 kDa band according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human Lung shows staining of lymphocytes in the vasculature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Carboxylesterase 1/CES1
Alternate Names
ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH
Entrez Gene IDs
1066 (Human)
Gene Symbol
CES1
UniProt
Additional Carboxylesterase 1/CES1 Products
Product Documents for Carboxylesterase 1/CES1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Carboxylesterase 1/CES1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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