Carboxymethyl Lysine Antibody

Catalog # Availability Size / Price Qty
MAB3247
MAB3247-SP
Detection of CML-adducted Protein by Western Blot.
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Product Details
Citations (10)
FAQs
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Reviews (1)

Carboxymethyl Lysine Antibody Summary

Specificity
Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.
Source
Monoclonal Mouse IgG2B Clone # 318003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Carboxymethyl Lysine-modified Keyhole Limpet Hemocyanin (KLH)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of CML-adducted Protein antibody by Western Blot. View Larger

Detection of CML-adducted Protein by Western Blot. Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxymethyl Lysine

N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.

Alternate Names
Carboxymethyl Lysine; CML

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Citations for Carboxymethyl Lysine Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Serial Passaging of RAW 264.7 Cells Modulates Intracellular AGE Formation and Downregulates RANKL-Induced In Vitro Osteoclastogenesis
    Authors: TT Lucy, ANM Mamun-Or-R, M Yagi, Y Yonei
    International Journal of Molecular Sciences, 2022;23(4):.
    Species: rack
    Sample Types:
  2. Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration
    Authors: HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman
    Cell Death & Disease, 2021;12(12):1128.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism
    Authors: L Senatus, R López-Díez, L Egaña-Gorr, J Liu, J Hu, G Daffu, Q Li, K Rahman, Y Vengrenyuk, TJ Barrett, MZ Dewan, L Guo, D Fuller, AV Finn, R Virmani, H Li, RA Friedman, EA Fisher, R Ramasamy, AM Schmidt
    JCI Insight, 2020;5(13):.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  4. Fructose liquid and solid formulations differently affect gut integrity, microbiota composition and related liver toxicity: a comparative in vivo study
    Authors: R Mastrocola, I Ferrocino, E Liberto, F Chiazza, AS Cento, D Collotta, G Querio, D Nigro, V Bitonto, JC Cutrin, K Rantsiou, M Durante, E Masini, M Aragno, C Cordero, L Cocolin, M Collino
    J. Nutr. Biochem., 2018;55(0):185-199.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Generation and characterization of mouse knockout for glyoxalase 1
    Authors: S Jang, DM Kwon, K Kwon, C Park
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. The Major Chromophore Arising from Glucose Degradation and Oxidative Stress Occurrence during Lens Proteins Glycation Induced by Glucose
    Authors: F Ávila, G Schmeda-Hi, E Silva
    Molecules, 2017;23(1):.
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.
    Authors: Mastrocola, Raffaell, Collino, Massimo, Nigro, Debora, Chiazza, Fausto, D'Antona, Giuseppe, Aragno, Manuela, Minetto, Marco A
    PLoS ONE, 2015;10(3):e0119587.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Increased protein aggregation in Zucker diabetic fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide.
    Authors: Talaei, Fatemeh, Van Praag, Veroniek, Shishavan, Mahdi H, Landheer, Sjoerd W, Buikema, Henk, Henning, Robert H
    BMC Cell Biol, 2014;15(0):1.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. An advanced glycation end product (AGE)-receptor for AGEs (RAGE) axis restores adipogenic potential of senescent preadipocytes through modulation of p53 protein function.
    Authors: Chen, Chih-Yu, Abell, Allison, Moon, Yang Soo, Kim, Kee-Hong
    J Biol Chem, 2012;287(53):44498-507.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Advanced glycation end products accumulate in vascular smooth muscle and modify vascular but not ventricular properties in elderly hypertensive canines.
    Authors: Shapiro BP, Owan TE, Mohammed SF, Meyer DM, Mills LD, Schalkwijk CG, Redfield MM
    Circulation, 2008;118(10):1002-10.
    Species: Canine
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Carboxymethyl Lysine Antibody

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Carboxymethyl Lysine Antibody
By Anonymous on 10/12/2021
Application: WB Sample Tested: Serum Species: Human