Carboxymethyl Lysine Antibody

(4 citations)   
  • Specificity
    Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 318003
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Carboxymethyl Lysine-modified Keyhole Limpet Hemocyanin (KLH)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CML-adducted Protein by Western Blot. Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxymethyl Lysine

N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.

  • Alternate Names:
    Carboxymethyl Lysine; CML
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.
    Authors: Mastrocola, Raffaell, Collino, Massimo, Nigro, Debora, Chiazza, Fausto, D'Antona, Giuseppe, Aragno, Manuela, Minetto, Marco A
    PLoS ONE, 2015;10(3):e0119587.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Increased protein aggregation in Zucker diabetic fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide.
    Authors: Talaei, Fatemeh, Van Praag, Veroniek, Shishavan, Mahdi H, Landheer, Sjoerd W, Buikema, Henk, Henning, Robert H
    BMC Cell Biol, 2014;15(0):1.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. An advanced glycation end product (AGE)-receptor for AGEs (RAGE) axis restores adipogenic potential of senescent preadipocytes through modulation of p53 protein function.
    Authors: Chen, Chih-Yu, Abell, Allison, Moon, Yang Soo, Kim, Kee-Hong
    J Biol Chem, 2012;287(53):44498-507.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Advanced glycation end products accumulate in vascular smooth muscle and modify vascular but not ventricular properties in elderly hypertensive canines.
    Authors: Shapiro BP, Owan TE, Mohammed SF, Meyer DM, Mills LD, Schalkwijk CG, Redfield MM
    Circulation, 2008;118(10):1002-10.
    Species: Canine
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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