CardioTACS In Situ Apoptosis Detection Kit

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues
Catalog # Availability Size / Price Qty
4827-30-K
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CardioTACS In Situ Apoptosis Detection Kit Summary

Provides researchers with an effective method for identifying apoptotic cells in cardiac samples using an optimized TUNEL based assay.

Key Benefits

• Performance tested on heart-derived samples.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when detecting apoptotic cardiac cells.

Why Use the CardioTACS In Situ Apoptosis Detection Kit?

The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system. R&D Systems has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label in apoptotic cells.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• Nuclear Fast Red
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• Cytonin

Kit Contents

  • Proteinase K
  • Streptavidin-HRP
  • TACS-Blue Label
  • Blue Strep-HRP Diluent
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • Nuclear Fast Red
  • TACS 2 TdT Labeling Buffer
  • TACS 2 TdT Stop Buffer
  • TACS 2 TdT dNTP
  • TdT Enzyme
  • 50x Manganese Cation
  • Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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