CardioTACS In Situ Apoptosis Detection Kit
CardioTACS In Situ Apoptosis Detection Kit Summary
• Performance tested on heart-derived samples.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when detecting apoptotic cardiac cells.
Why Use the CardioTACS In Situ Apoptosis Detection Kit?
The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system. R&D Systems has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label in apoptotic cells.
/////Proteinase K/////Streptavidin-HRP/////TACS-Blue Label/////Blue Strep-HRP Diluent/////TACS-Nuclease/////TACS-Nuclease Buffer/////Nuclear Fast Red/////TACS 2 TdT Labeling Buffer/////TACS 2 TdT Stop Buffer/////TACS 2 TdT dNTP/////TdT Enzyme/////50x Manganese Cation/////Cytonin
For research use only. Not for diagnositic use.
Visualization of an Apoptotic Rat Cardiac Myocyte using the CardioTACS In Situ Apoptosis Detection Kit. Rat heart tissue was fixed in 4% formaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. Samples were processed following the CardioTACS Kit protocol (Catalog # 4827-30-K) and visualized by light microscopy. Photo was provided courtesy of Dr. J. Zhang, FDA.
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