CardioTACS In Situ Apoptosis Detection Kit

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cardiac cells and tissues
Catalog # Availability Size / Price Qty
4827-30-K
CardioTACS In Situ Apoptosis Detection Kit_Rat Heart Tissue_4827-30-K
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Product Details
Citations (14)
FAQs
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CardioTACS In Situ Apoptosis Detection Kit Summary

Provides researchers with an effective method for identifying apoptotic cells in cardiac samples using an optimized TUNEL based assay.

Key Benefits

• Performance tested on heart-derived samples.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when detecting apoptotic cardiac cells.

Why Use the CardioTACS In Situ Apoptosis Detection Kit?

The high cellularity of cardiac tissue presents problems in permeabilization, so the CardioTACS Kit comes with two permeabilization reagents to provide options. The kit is based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system. R&D Systems has developed an exclusive apoptosis grade TdT enzyme that when coupled with the TACS Blue Label solution, provides labeling 20 to 50 times more sensitive than standard diaminobenzidine (DAB) labeling methods. To ensure ease of data interpretation when the numbers of apoptotic cells are low, CardioTACS includes Nuclear Fast Red Counterstain which provides superb contrast to the TACS Blue Label in apoptotic cells.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• Nuclear Fast Red
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Visualization of an Apoptotic Rat Cardiac Myocyte using the CardioTACS In Situ Apoptosis Detection Kit. Rat heart tissue was fixed in 4% formaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. Samples were processed following the CardioTACS Kit protocol (Catalog # 4827-30-K) and visualized by light microscopy. Photo was provided courtesy of Dr. J. Zhang, FDA.

Reagents Provided

Citations for CardioTACS In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Effects of low-dose oxygen ions and protons on cardiac function and structure in male C57BL/6J mice
    Authors: JW Seawright, V Sridharan, RD Landes, M Cao, P Singh, I Koturbash, XW Mao, IR Miousse, SP Singh, GA Nelson, M Hauer-Jens, M Boerma
    Life Sci Space Res (Amst), 2019;20(0):72-84.  2019
  2. Reversal of cardiac, vascular, and renal dysfunction by non-quinazoline ?1-adrenolytics in DOCA-salt hypertensive rats: a comparison with prazosin, a quinazoline-based ?1-adrenoceptor antagonist
    Authors: M Kubacka, M Zadro?na, B Nowak, M Kota?ska, B Filipek, AM Waszkielew, H Marona, S Mogilski
    Hypertens. Res., 2019;0(0):.  2019
  3. REV-ERB? ameliorates heart failure through transcription repression
    Authors: L Zhang, R Zhang, CL Tien, RE Chan, K Sugi, C Fu, AC Griffin, Y Shen, TP Burris, X Liao, MK Jain
    JCI Insight, 2017;2(17):.  2017
  4. IGF-1 degradation by mouse mast cell protease 4 promotes cell death and adverse cardiac remodeling days after a myocardial infarction
    Authors: Thor Tejada
    Proc Natl Acad Sci USA, 2016;0(0):.  2016
  5. Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
    Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
    Lab Invest, 2012;92(11):1518-26.  2012
  6. Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.
    Authors: Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
    J. Am. Coll. Cardiol., 2010;55(2):135-43.  2010
  7. Targeted cardiac expression of soluble Fas prevents the development of heart failure in mice with cardiac-specific expression of MCP-1.
    Authors: Niu J, Azfer A, Deucher MF, Goldschmidt-Clermont PJ, Kolattukudy PE
    J. Mol. Cell. Cardiol., 2006;40(6):810-20.  2006
  8. Tumor necrosis factor receptor deficiency exacerbated Adriamycin-induced cardiomyocytes apoptosis: an insight into the Fas connection.
    Authors: Lien YC, Lin SM, Nithipongvanitch R, Oberley TD, Noel T, Zhao Q, Daosukho C, St Clair DK
    Mol. Cancer Ther., 2006;5(2):261-9.  2006
  9. Probenecid as a noninjurious positive inotrope in an ischemic heart disease murine model.
    Authors: Koch S, Tranter M, Robbins N, Luther K, Singh U, Jiang M, Ren X, Tee T, Smith L, Varma P, Jones W, Rubinstein J
    J Cardiovasc Pharmacol Ther, 0;18(3):280-9.  0
  10. The influence of age on serum concentrations of cardiac troponin I: results in rats, monkeys, and commercial sera.
    Authors: Herman E, Knapton A, Liu Y, Lipshultz S, Estis J, Todd J, Woodward R, Cochran T, Zhang J, Poirier M
    Toxicol Pathol, 0;42(5):888-96.  0
  11. Silencing of p53 RNA through transarterial delivery ameliorates renal tubular injury and downregulates GSK-3beta expression after ischemia-reperfusion injury.
    Authors: Fujino T, Muhib S, Sato N, Hasebe N
    Am J Physiol Renal Physiol, 0;305(11):F1617-27.  0
  12. GSK-3beta heterozygous knockout is cardioprotective in a knockin mouse model of familial dilated cardiomyopathy.
    Authors: Mohamed R, Morimoto S, Ibrahim I, Zhan D, Du C, Arioka M, Yoshihara T, Takahashi-Yanaga F, Sasaguri T
    Am J Physiol Heart Circ Physiol, 0;310(11):H1808-15.  0
  13. Phosphodiesterase 5 Inhibition Limits Doxorubicin-induced Heart Failure by Attenuating Protein Kinase G Ialpha Oxidation.
    Authors: Prysyazhna O, Burgoyne J, Scotcher J, Grover S, Kass D, Eaton P
    J Biol Chem, 0;291(33):17427-36.  0
  14. A multifaceted evaluation of imatinib-induced cardiotoxicity in the rat.
    Authors: Herman E, Knapton A, Rosen E, Thompson K, Rosenzweig B, Estis J, Agee S, Lu Q, Todd J, Lipshultz S, Hasinoff B, Zhang J
    Toxicol Pathol, 0;39(7):1091-106.  0

FAQs

  1. Once the frozen reagents supplied in the Cardio TACS™ In Situ Apoptosis Detection Kit, Catalog # 4827-30-K, are thawed, how should they be stored?

    • Frozen reagents supplied in the Cardio TACS™ In Situ Apoptosis Detection Kit, Catalog # 4827-30-K, should be stored frozen in the original vial after use. These reagents, if handled as instructed in the kit booklet, can undergo multiple freeze thaw cycles provided it is within the kit expiraiton data.

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