Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

Novus Biologicals | Catalog # NBP2-52449

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F12F3

Format

BSA Free
View all Casein Kinase 2 beta Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Casein Kinase 2 beta (AA: FULL(1-215)) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), K562 (3), HepG2 (4), C6 (5), SK-N-SH (6), NTERA-2 (7), MCF-7 (8), NIH/3T3 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunocytochemistry/Immunofluorescence: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of MCF-7 cells using CSNK2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of paraffin-embedded colon cancer tissues using CSNK2B mouse mAb with DAB staining.
Flow Cytometry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Flow Cytometry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Flow Cytometry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Flow cytometric analysis of Hela cells using CSNK2B mouse mAb (green) and negative control (red).
Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mAb against HEK293 (1) and CSNK2B (AA: FULL(1-215))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3)BSA Free [NBP2-52449]

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mAb against human CSNK2B (AA: FULL(1-215)) recombinant protein. (Expected MW is 35 kDa)
Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of paraffin-embedded cervical cancer tissues using CSNK2B mouse mAb with DAB staining.
ELISA: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

ELISA: Casein Kinase 2 beta Antibody (2F12F3) - BSA Free [NBP2-52449]

ELISA: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Casein Kinase 2 beta

Casein Kinase 2 beta encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Alternate Names

CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin

Gene Symbol

CSNK2B

Additional Casein Kinase 2 beta Products

Product Documents for Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
Notch Pathway

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail