Caspase-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56564

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-1 Primary Antibodies »

Product Specifications

Immunogen

Caspase-1 Antibody was developed against a synthetic peptide made to an internal portion of the human Caspase-1 protein (between amino acids 15-60) [UniProt P29466].

Reactivity Notes

Porcine (85%), Rabbit (85%) and Canine (70%).

Specificity

Caspase-1 Antibody recognizes full-length Caspase-1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-1 Antibody - BSA Free

Knockout Validated: Caspase-1 Antibody [NB100-56564]

Western Blot: Caspase-1 Antibody [NB100-56564]

Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.
Western Blot: Caspase-1 Antibody [NB100-56564]

Western Blot: Caspase-1 Antibody [NB100-56564]

Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564]

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564]

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.
Caspase-1 Antibody - BSA Free

Western Blot: Caspase-1 Antibody - BSA Free [NB100-56564] -

Sevoflurane can increase the protein expression of NLRP3 inflammasome and its downstream inflammatory factors in prefrontal cortex. A–E Expression levels of NLRP3 inflammatory factors, related components, and downstream cytokine proteins. Western blot images were cropped to remove irrelevant sections of the image and display only the proteins of interest. Loading control: protein extracted from prefrontal cortex tissue from sham subjects treated with saline (lane1). Tissue used for Western blot analysis was collected 24-h after the last drug-administration. F–I NLRP3 inflammatory factors, related components, and downstream cytokine PCR results. Data are expressed as mean +/- SD; n = 4 for each experimental group. ***Compared with the Ctrl group, ###compared with the Sev group. P < 0.05 indicated a statistically significant difference Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36709248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Caspase-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-1

Caspase-1, also known as interleukin-1-beta (IL-1beta) -converting enzyme (ICE), is a member of the cysteine-aspartic protease (caspase) family (1). The caspase family are aspartic proteolytic enzymes that play a key role in apoptotic cell death and inflammation (1). Caspase-1 is synthesized as an inactive proenzyme with a theoretical molecular weight of approximately 45 kDa and is 404 amino acids (aa) in length. The caspase-1 protein structure contains an N-terminal CARD (caspase recruitment domain), a large 20kDa P20 subunit (aa 120-297), and a small 10kDa P10 subunit (aa 317-404) (1, 2). The proenzyme undergoes cleavage at aspartic acid (Asp) residues to produce the active caspase-1 enzyme which exists as a heterotetramer (homodimer of the P20 and P10 heterodimers) (2). Inflammasome complexes sense various microbial and endogenous signals, including ligand-receptor interaction and growth factor deprivation, which results in the processing of pro-caspase to active caspase-1 (3, 4).The active caspase-1 is responsible for the cleavage and maturation of pro-inflammatory cytokines IL-1beta and IL-18, and promotes an inflammatory form of cell death induced by bacterial pathogens called pyroptosis (3, 4). Mature IL-1beta has a role in the immune reaction and recruiting inflammatory cells to the site of infection while IL-18 plays a role in interferon-gamma production and promotes cytolytic activity in natural killer cells (4).

Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.

Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC 3.4.22.36, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.

References

1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation. https://doi.org/10.1038/cdd.2014.216

2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR. https://doi.org/10.1128/mmbr.64.4.821-846.2000

3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology. https://doi.org/10.1016/j.tcb.2014.12.009

4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology. https://doi.org/10.1038/ni.1703

5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews. https://doi.org/10.1111/imr.12906

6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-020-01274-7

Alternate Names

CASP1, Caspase1, ICE

Entrez Gene IDs

834 (Human)

Gene Symbol

CASP1

UniProt

P29466 (Human)

Additional Caspase-1 Products

Product Documents for Caspase-1 Antibody - BSA Free

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Product Specific Notices for Caspase-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspase-1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-1 Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: We are interested in your item, NB100-56564 (IMG-5025) and would like to confirm if it only recognizes subunit p10 and do not cross react to p20?

    A: Our Caspase 1 antibody with catalogue number NB100-56564 (IMG-5025) was raised against a synthetic peptide corresponding to amino acids 371-390 of the human protein. According to the Uniprot listing these amino acids lie within the p10 subunit. The antibody recognises full-length Caspase-1, as well as cleaved Caspase-1 forms that include amino acids 31-45. Amino acids 31-45 lie within the propeptide. Unfortunately we have no testing data where we have used this antibody to probe the isolated p20 subunit. Based on the immunogen sequence it seems unlikely that the antibody would recognise p20, but I unfortunately do not have 100% certainty on this at the present time.

  • Q: We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of Caspase-1. Would you please let me know if NB100-56565 and NB100-56564 recognize the Caspase-1 p10 or p20? If so, could you please provide me any supporting data of them?

    A:

    For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit, I would recommend NBP1-45433. This will bind to the the region around aa 132. The p20 subunit is aa 120-297.

  • Q: We are interested in your item, NB100-56564 (IMG-5025) and would like to confirm if it only recognizes subunit p10 and do not cross react to p20?

    A: Our Caspase 1 antibody with catalogue number NB100-56564 (IMG-5025) was raised against a synthetic peptide corresponding to amino acids 371-390 of the human protein. According to the Uniprot listing these amino acids lie within the p10 subunit. The antibody recognises full-length Caspase-1, as well as cleaved Caspase-1 forms that include amino acids 31-45. Amino acids 31-45 lie within the propeptide. Unfortunately we have no testing data where we have used this antibody to probe the isolated p20 subunit. Based on the immunogen sequence it seems unlikely that the antibody would recognise p20, but I unfortunately do not have 100% certainty on this at the present time.

  • Q: We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of Caspase-1. Would you please let me know if NB100-56565 and NB100-56564 recognize the Caspase-1 p10 or p20? If so, could you please provide me any supporting data of them?

    A:

    For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit, I would recommend NBP1-45433. This will bind to the the region around aa 132. The p20 subunit is aa 120-297.

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