Caspase-14 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56126

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Gerbil

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-14 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Caspase-14 was used as immunogen.

Specificity

This polyclonal antibody recognizes the proform of caspase-14 (~-28-32 kDa), and the large (~14-21 kDa) and small (~10-11 kDa) of active/cleaved caspase-14.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-14 Antibody - BSA Free

Western Blot: Caspase-14 Antibody [NB100-56126]

Western Blot: Caspase-14 Antibody [NB100-56126]

Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.
Immunohistochemistry: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry: Caspase-14 Antibody [NB100-56126] - Tissue sections of mouse skin at E17 stained using this antibody at 1:500.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Human ovarian cancer tissue microarray stained for Caspase-14 expression using this antibody at 1:2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1). Decreased Caspase-14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126]

Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Tissue sections of human cervix stained using this antibody at 1:2000. A. Normal cervix (squamous epithelium). B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia). C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia. D. CIN3 (high-grade squamous intraepithelial lesion; severe dysplasia-carcinoma in situ. In normal cervi, caspase-14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of caspase-14 expression with differentiation. Caspase-14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.

Applications for Caspase-14 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-14

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-14 activation has been implicated in keratinocyte senescence which leads to the cornified cell layer, suggesting a role for caspase-14 in epithelial cell differentiation (Pistritto et al. 2002). Terminal differentiation of keratinocytes has some features of classical apoptosis including DNA fragmentation, nuclear condensation, and induction of cross-linking transglutaminases. Tumor-specific alterations in caspase-14 expression have been found in epithelial malignancies, suggesting that caspase-14 plays a role in epithelial cell transformation. The caspase-14 NB100-56126 antibody recognizes the proform of caspase-14 (approx. 28-32 kDa), and the large (approx. 14-21 kDa) and small (approx. 10-11 kDa) of active/cleaved caspase-14.

Alternate Names

CASP14, Caspase14

Entrez Gene IDs

23581 (Human); 12365 (Mouse); 299587 (Rat)

Gene Symbol

CASP14

UniProt

NP_033939 (Mouse)

Additional Caspase-14 Products

Product Documents for Caspase-14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-14 Antibody - BSA Free

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Protocols

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