Caspase-3 Antibody (31A893) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80613

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 31A893

Format

Azide and BSA Free
View all Caspase-3 Primary Antibodies »

Product Specifications

Immunogen

This Caspase-3 Antibody (31A893) - Azide and BSA Free was developed against recombinant full-length human Caspase-3 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-3 Antibody (31A893) - Azide and BSA Free

Western Blot: Caspase-3 Antibody (31A893)Azide and BSA Free [NBP2-80613]

Western Blot: Caspase-3 Antibody (31A893)Azide and BSA Free [NBP2-80613]

Western Blot: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613] - Analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613] - Left panel: Untreated Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with Caspase-3 Antibody (31A893) at 10 ug/mL overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 5 ug/mL of mouse monoclonal Caspase-3 Antibody (31A893) (NB100-56709, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte
Western Blot: Caspase-3 Antibody (31A893)Azide and BSA Free [NBP2-80613]

Western Blot: Caspase-3 Antibody (31A893)Azide and BSA Free [NBP2-80613]

Western Blot: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613] - Analysis for human Caspase-3 using HL60 lysates with Caspase-3 Antibody (31A893) at 2 ug/mL dilution. NB100-56709 only detects a 32 kD Caspase-3 corresponding to pro-Caspase-3. Image from the standard format of this antibody.
Immunohistochemistry: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunohistochemistry: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613]

Immunohistochemistry: Caspase-3 Antibody (31A893) - Azide and BSA Free [NBP2-80613] - Tissue section of normal human breast using 5 ug/mL concentration of Caspase-3 Antibody (31A893). Very strong diffused as well as granular immunopositivity of Caspase 3 was observed specifically in the cytoplasmic of ductal /acinar epithelial cells. Image

Applications for Caspase-3 Antibody (31A893) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 ug/ml
Application Notes
For IHC-P validation of this target, 1M EDTA pH 9.0 buffer was used for antigen retrieval (citrate buffer pH 6.0 did not work).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-3

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G.,... Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y.,... Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A.,... Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Antibody (31A893) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-3 Antibody (31A893) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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