Caspase-7 Antibody (25B881.1) - BSA Free

Novus Biologicals | Catalog # NB100-56529

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 25B881.1

Format

BSA Free
View all Caspase-7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated against recombinant protein of active form of human Caspase-7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Caspase-7 Antibody (25B881.1) - BSA Free

Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]

Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]

Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]

Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]

Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]

Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]

Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Applications for Caspase-7 Antibody (25B881.1) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
In Jurkat, a 35 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-7

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.

Alternate Names

CASP7, Caspase7, Mch3

Entrez Gene IDs

840 (Human)

Gene Symbol

CASP7

UniProt

P55210 (Mouse)

Additional Caspase-7 Products

Product Documents for Caspase-7 Antibody (25B881.1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-7 Antibody (25B881.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-7 Antibody (25B881.1) - BSA Free

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail