Caspase-8 Antibody (1H11)

Novus Biologicals | Catalog # NBP2-22183

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H11
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Caspase-8 expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-8 Antibody (1H11)

Western Blot: Caspase-8 Antibody (1H11)BSA Free [NBP2-22183]

Western Blot: Caspase-8 Antibody (1H11)BSA Free [NBP2-22183]

Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of liver cancer tissues using Caspase 8 mouse mAb with DAB staining.
Flow Cytometry: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Flow Cytometry: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Flow Cytometry: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of NIH/3T3 cells using Caspase 8 mouse mAb (blue) and negative control (red).
Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of colon cancer tissues using Caspase 8 mouse mAb with DAB staining.
ELISA: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

ELISA: Caspase-8 Antibody (1H11) - BSA Free [NBP2-22183]

ELISA: Caspase-8 Antibody (1H11) [NBP2-22183] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for Caspase-8 Antibody (1H11)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-8

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (1H11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caspase-8 Antibody (1H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Caspase-8 Antibody (1H11)

There are currently no reviews for this product. Be the first to review Caspase-8 Antibody (1H11) and earn rewards!

Have you used Caspase-8 Antibody (1H11)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 Antibody (1H11)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies

Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail