Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Caspase-8 Antibody

Novus Biologicals | Catalog # NB600-576

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Recombinant full length human Caspase 8 protein.

Localization

Cytoplasmic

Specificity

This reacts with a 55 kDa protein. Caspase 8 has an N-terminal domain with sequence homology to the death effector domain (DED) of FADD. Caspase 8 binds to DED of FADD. Caspase 8 is activated by association with Fas/FADD death-inducing signaling complex to release two active subunits, p18 and p10 into the cytosol, where they activate other caspases amplifying the apoptotic signal.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-8 Antibody

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576]

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576] - Formalin fixed paraffin embedded human stomach stained with Caspase-8 antibody (NB600-576).

Immunohistochemistry: Caspase-8 Antibody [NB600-576]

Immunohistochemistry: Caspase-8 Antibody [NB600-576] - Formalin-fixed, paraffin-embedded human stomach stained with Caspase 8. Note cytoplasmic staining of epithelial cells.

Applications for Caspase-8 Antibody

Application

Recommended Usage

Immunohistochemistry

1:50-1:100

Application Notes

IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Western Blot was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Caspase-8

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Alternate Names

CASP8, Caspase8, Mch5

Entrez Gene IDs

841 (Human); 12370 (Mouse); 64044 (Rat)

Gene Symbol

CASP8

UniProt

Q14790

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Product Documents for Caspase-8 Antibody

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Certificate of Analysis

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Product Specific Notices for Caspase-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

RIG-I-like Receptors

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