Caspase-9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56118

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Gerbil

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Flow Cytometry Control, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-9 Primary Antibodies »

Product Specifications

Immunogen

Human Caspase 9 recombinant protein catalytic subunits (NP_001220).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-9 Antibody - BSA Free

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0014.jpg
Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0013.jpg
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.
Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry: Caspase-9 Antibody [NB100-56118]

Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0012.jpg
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a dog brain 2 hr after reperfusion injury. B) ection from a dog brain sham control (brain surgery but no injury). Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118]

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a patient 24 hr after head trauma. B) Control: section from a patient with no known neurological disease or head injury. Hematoxylin-eosin counterstain.
Caspase-9 Antibody

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NB100-56118] -

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis & altered inflammation in double-deficient Atg7 delta pan-Rip3−/− mice. (a) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-3 in 12-week-old mice. Caspase-3 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-active caspase-3 ab2302, 1/50, scale bar=50 μm). (b) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic Bax in 12-week-old mice. Bax quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-Bax (sc-526, 1/50). (c) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-9 (red) (anti-caspase-9 NB100-56118, 1/1000). (d) Reduced infiltration inflammation of macrophages (F4/80, NBP2-12506, 1/75) & early T-lymphocytes (MPO, ab9535, 1/50) in double-deficient Atg7 delta pan-Rip3d/d (n=10) mice compared with pancreatic Atg7 delta pan (n=7). Data are mean±S.E.M. for the numbers of animals as indicated in the graph, *P<0.05, **P<0.01, NS = no significance Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28703808), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caspase-9 Antibody - BSA Free

Western Blot: Caspase-9 Antibody - BSA Free [NB100-56118] -

Nuclear translocation of TCA cycle enzymes prevents doxorubicin-mediated cellular damage.a–c Enzyme activity of IDH-2 (n = 4) (a), MDH-2 (n = 3) (b), and PDHC (n = 3) (c) in nuclei isolated from cells transduced with tetracycline-inducible NLS-IDH-2 constructs. Activity is represented as the rate in nM/min or delta OD/min, p value* <0.05, p value**** = 0.0001, and lysates were probed for PCNA to detect equal nuclear protein amounts used for the assay. d Reduced apoptotic cell death as measured by Annexin:V:FITC staining in cells expressing nuclear TCA cycle dehydrogenases prior to doxorubicin DRN treatment. N = 3 p value* <0.05 (e) Quantification of troponin I released from cardiomyocytes as a marker of cardiac injury, minimum n = 4 p value** <0.007, p value* <0.05. f Cell death as measured by Cell Titer GloTM in cells expressing nuclear dehydrogenases prior to DRN treatment. minimum n = 4 p value*** = 0.0001, p value**** <0.0001. NLS-EGFP-2A-NLS-mCherry was used as a control for the 2 A linker system. g DNA damage as assessed by ɣ-H2AX foci formation. Scale bar 20 um. h, i qRT‒PCR showing the expression of anti-apoptotic genes BCL2 (h) and MCL1 (i). n = 3 P value ** <0.005, *<0.05. j–l Protein levels of anti-apoptotic proteins (j, k along with reduced caspase 9 cleavage (l) in cells expressing nuclear IDH-2 compared to cells treated with doxorubicin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37468519), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Caspase-9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 22223358)

Immunohistochemistry-Frozen

Assay Dependent

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

reported in scientific literature (PMID 24184144)
Application Notes
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active/cleaved Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation: the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.

Reviewed Applications

Read 1 review rated 5 using NB100-56118 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-9

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Alternate Names

CASP9, Caspase9

Entrez Gene IDs

842 (Human); 58918 (Rat)

Gene Symbol

CASP9

UniProt

P55211

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-9 Antibody - BSA Free

Customer Reviews for Caspase-9 Antibody - BSA Free (1)

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Customer Images

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  • Name: Chi Li
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Transplanted lung tumor section
    Species: Mouse
    Verified Customer | Posted 01/08/2015
    Section of transplanted lung tumors from mouse
    Caspase-9 Antibody - BSA Free NB100-56118

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FAQs for Caspase-9 Antibody - BSA Free

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  • Q: Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?

    A: After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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