Catalase Antibody (OTI1B8)

Novus Biologicals | Catalog # NBP2-00492

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8
View all Catalase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CAT (NP_111743) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Peroxisome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Catalase Antibody (OTI1B8)

Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunocytochemistry/ Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunocytochemistry/Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492] - Mouse hepatocytes stained with catalase antibody. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.
Flow Cytometry: Catalase Antibody (OTI1B8) [NBP2-00492]

Flow Cytometry: Catalase Antibody (OTI1B8) [NBP2-00492]

Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Catalase Antibody (OTI1B8) [NBP2-00492]

Western Blot: Catalase Antibody (OTI1B8) [NBP2-00492]

Western Blot: Catalase Antibody (1B8) [NBP2-00492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Catalase.
Immunocytochemistry/ Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunocytochemistry/ Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunocytochemistry/Immunofluorescence: Catalase Antibody (1B8) [NBP2-00492] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.
Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (OTI1B8) [NBP2-00492]

Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.
Flow Cytometry: Catalase Antibody (OTI1B8) [NBP2-00492]

Flow Cytometry: Catalase Antibody (OTI1B8) [NBP2-00492]

Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Catalase Antibody (OTI1B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 1 review rated 5 using NBP2-00492 in the following applications:

Flow Cytometry Panel Builder

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  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Catalase

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq].

Alternate Names

Cas1, CAT, Cs-1

Entrez Gene IDs

847 (Human)

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Catalase Antibody (OTI1B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Catalase Antibody (OTI1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Oxidative Stress

Citations for Catalase Antibody (OTI1B8)

Customer Reviews for Catalase Antibody (OTI1B8) (1)

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Customer Images

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  • Catalase Antibody (OTI1B8)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Hepatocytes
    Species: Mouse
    Verified Customer | Posted 11/05/2021
    Catalase Antibody in hepatocytes
    Catalase Antibody (OTI1B8) NBP2-00492

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