Catenin alpha 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15723

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Rabbit (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Catenin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Catenin alpha 1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (86%).

Localization

Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Catenin alpha 1 Antibody - BSA Free

Western Blot: Catenin alpha 1 Antibody [NBP2-15723]

Western Blot: Catenin alpha 1 Antibody [NBP2-15723]

Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] diluted at 1:500.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723]

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723]

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N1N3] dilution: 1:500.
Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723]

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723]

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] - Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N1N3] dilution: 1:500.
Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723]

Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723]

Immunoprecipitation: Catenin alpha 1 Antibody [NBP2-15723] - IP samples: HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of CTNNA1 protein by 4 ug alpha 1 Catenin antibody [N1N3] 5 % SDS-PAGE The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Catenin alpha 1 Antibody

Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -

Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:500
Catenin alpha 1 Antibody

Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -

Western Blot: Catenin alpha 1 Antibody [NBP2-15723] -Sample (30 ug of whole cell lysate), A: 293T, B: A431, C: HeLa 5% SDS PAGE diluted at 1:1000
Catenin alpha 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NBP2-15723] -

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (NBP2-15723) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Catenin alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Catenin alpha 1

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Alternate Names

Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13

Gene Symbol

CTNNA1

UniProt

P35221 (Human)

Additional Catenin alpha 1 Products

Product Documents for Catenin alpha 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Catenin alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for Catenin alpha 1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Catenin alpha 1 Antibody - BSA Free and earn rewards!

Have you used Catenin alpha 1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies