Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89728

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free (NBP1-89728) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunocytochemistry/ Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining in human kidney and skeletal muscle tissues. Corresponding CTSA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human skeletal muscle shows only very weak positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.
Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free [NBP1-89728]

Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free [NBP1-89728]

Analysis in human cell line U-87 MG.

Applications for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Entrez Gene IDs

5476 (Human)

Gene Symbol

CTSA

UniProt

P10619

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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