Cathepsin L/V/K/H Antibody (JM10-78)

Novus Biologicals | Catalog # NBP2-67216

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM10-78 expressed in HEK293
View all Cathepsin L/V/K/H Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Cathepsin L/V/K/H aa 92-138 / 333. (SwissProt: P07711 Human; SwissProt: O60911 Human; SwissProt: P43235 Human; SwissProt: P09668 Human)

Localization

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Nucleus, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin L/V/K/H Antibody (JM10-78)

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate
Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.
Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549
Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.
Cathepsin L/V/K/H Antibody (JM10-78) Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]

Western blot analysis of HCT 116 cell lysate with NBP2-67216 at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67216, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37/38 kDa Observed band size: 56 kDa

Applications for Cathepsin L/V/K/H Antibody (JM10-78)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin L/V/K/H

Alternate Names

ACC4, ACC-4, ACC5, ACC-5, aleurain, cathepsin B3, cathepsin BA, cathepsin K, cathepsin L1, cathepsin L2, cathepsin L2, preproprotein, Cathepsin O, cathepsin O1, Cathepsin O2, Cathepsin U, Cathepsin X, CATL, CATL2, CPSB, CTS02, CTSL1, CTSL2, CTSO, CTSO1, CTSO2, CTSU, Major excreted protein, MEP, N-benzoylarginine-beta-naphthylamide hydrolase, PKND, procathepsin L, PYCD

Gene Symbol

CTSL

Additional Cathepsin L/V/K/H Products

Product Documents for Cathepsin L/V/K/H Antibody (JM10-78)

Certificate of Analysis

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Product Specific Notices for Cathepsin L/V/K/H Antibody (JM10-78)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cathepsin L/V/K/H Antibody (JM10-78)

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Protocols

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