Caveolin-1 Antibody (7C8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80617

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Sheep

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7C8

Format

Azide and BSA Free
View all Caveolin-1 Primary Antibodies »

Product Specifications

Immunogen

Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caveolin-1 Antibody (7C8) - Azide and BSA Free

Western Blot: Caveolin-1 Antibody (7C8)Azide and BSA Free [NBP2-80617]

Western Blot: Caveolin-1 Antibody (7C8)Azide and BSA Free [NBP2-80617]

Western Blot: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-1…), licensed under a CC-BY license. Image from the standard format of this antibody.
Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.
Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617]

Flow Cytometry: Caveolin-1 Antibody (7C8) - Azide and BSA Free [NBP2-80617] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin.

Applications for Caveolin-1 Antibody (7C8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24758774)

Immunohistochemistry-Paraffin

1:100 - 1:300

Western Blot

1:1000 - 1:4000
Application Notes
In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caveolin-1

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Gene Symbol

CAV1

Additional Caveolin-1 Products

Product Documents for Caveolin-1 Antibody (7C8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Caveolin-1 Antibody (7C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caveolin-1 Antibody (7C8) - Azide and BSA Free

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Protocols

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