Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Sheep

Cited:

Human, Mouse, Rat, Insect - Drosophila melanogaster, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7C8

Format

BSA Free
View all Caveolin-1 Primary Antibodies »

Product Specifications

Immunogen

Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes

Localization

Cell Membrane

Marker

Caveolae Marker, Endosome Marker

Specificity

This is specific for caveolin alpha and beta proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caveolin-1 Antibody (7C8) - BSA Free

Western Blot: Caveolin-1 Antibody (7C8) [NB100-615]

Western Blot: Caveolin-1 Antibody (7C8) [NB100-615]

Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0014.jpg
Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615]

Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615]

Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615]

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615]

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Applications for Caveolin-1 Antibody (7C8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunoblotting

reported in scientific literature (PMID 31759628)

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24758774)

Immunohistochemistry-Paraffin

1:100-1:300

Immunoprecipitation

1-2 ug / 500 ug of protein

Western Blot

1:1000-1:4000
Application Notes
In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used.

Reviewed Applications

Read 3 reviews rated 5 using NB100-615 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caveolin-1

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Gene Symbol

CAV1

Additional Caveolin-1 Products

Product Documents for Caveolin-1 Antibody (7C8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caveolin-1 Antibody (7C8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caveolin-1 Antibody (7C8) - BSA Free

Customer Reviews for Caveolin-1 Antibody (7C8) - BSA Free (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Caveolin-1 Antibody (7C8)
    Name: auriane ernault
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adipose tissue
    Species: Human
    Verified Customer | Posted 03/05/2021
    Immunofluorescence of human adipose tissue
    Antibody dilution 1:250
    Caveolin-1 Antibody (7C8) - BSA Free NB100-615
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 08/12/2015
    Immunocytochemistry of caveolin 1
    Caveolin-1 Antibody (7C8) - BSA Free NB100-615
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID: 24348251
    Species: Human
    Verified Customer | Posted 12/19/2014

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Protocols

View specific protocols for Caveolin-1 Antibody (7C8) - BSA Free (NB100-615):


Western Blot Protocol

1. Perform SDS-PAGE (4-12%) on samples to be analyzed, loading 50ug of total protein per lane.

2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.

3. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.

4. Rinse the blot in TBS for approximately 5 minutes.

5. Block the membrane using 5% non-fat dry milk in TBS for 1 hour.

6. Dilute the mouse anti-Caveolin primary antibody (NB 100-615) in blocking buffer and incubate 3 hours at room temperature.

7. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.

8. Wash the blot in TBS containing 0.05-0.1% Tween-20 for 10-20 minutes.

9. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).

10. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used at Novus Biologicals).

Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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FAQs for Caveolin-1 Antibody (7C8) - BSA Free

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  • Q: What is the sodium azide percentage for this product?

    A: 0.05% sodium azide 

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