Caveolin-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31116

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chimpanzee (100%), Feline (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caveolin-2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin-2. The exact sequence is proprietary.

Reactivity Notes

Cat (100%).

Localization

Golgi apparatus membrane, Single-pass membrane protein, Cell membrane, Single-pass membrane protein, Membrane, caveola, Single-pass membrane protein, Golgi apparatus membrane, Cell membrane, Membrane, caveola

Marker

Caveolae Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caveolin-2 Antibody - BSA Free

Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116]

Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116]

Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116] - FaDu xenograft, using Caveolin 2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116]

Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116]

Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ug Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Caveolin-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] -

Caveolin-2 antibody detects Caveolin-2 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin-2 protein stained by Caveolin-2 antibody (NBP1-31116) diluted at 1:500.
Blue: Hoechst 33342 staining.
Caveolin-2 Antibody - BSA Free

Western Blot: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

The S100 protein family affects HNSCC metastasis and is regulated by CAV2.A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caveolin-2 Antibody - BSA Free

Knockdown Validated: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

CAV2 promotes HNSCC metastasis both in vitro and in vivo.Silencing CAV2 in SCC15 and SCC25 cells by transfection with two individual CAV2 shRNAs significantly decreased CAV2 expression, as detected by western blotting (A) and QT-PCR (B). C, D The silencing of CAV2 significantly inhibited the migration and invasion of SCC15 and SCC25 cells, as evaluated using Transwell assays. (Scale bar, 200 μm). E Images of the macroscopic lung tissues of tail vein-injected mice. F The lung metastasis nodules were counted, and the data from the shCAV2 and negative control groups are summarized. ‘****’ indicates ‘p < 0.0001’. G CAV2 IHC staining and H&E staining to assess lung metastasis in tail vein-injected mice. (Scale bars, 200 μm and 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caveolin-2 Antibody - BSA Free

Immunohistochemistry: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

High expression of CAV2 is associated with the poor prognosis of HNSCC patients.A IHC analysis of CAV2 levels in 211 human HNSCC samples (scale bar, 100 μm or 400 μm). B Kaplan–Meier survival curve showing the correlation of overall survival and disease-free survival with the CAV2 IHC score. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caveolin-2 Antibody - BSA Free

Immunohistochemistry: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

CAV2 promotes HNSCC metastasis both in vitro and in vivo.Silencing CAV2 in SCC15 and SCC25 cells by transfection with two individual CAV2 shRNAs significantly decreased CAV2 expression, as detected by western blotting (A) and QT-PCR (B). C, D The silencing of CAV2 significantly inhibited the migration and invasion of SCC15 and SCC25 cells, as evaluated using Transwell assays. (Scale bar, 200 μm). E Images of the macroscopic lung tissues of tail vein-injected mice. F The lung metastasis nodules were counted, and the data from the shCAV2 and negative control groups are summarized. ‘****’ indicates ‘p < 0.0001’. G CAV2 IHC staining and H&E staining to assess lung metastasis in tail vein-injected mice. (Scale bars, 200 μm and 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Caveolin-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31116 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caveolin-2

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins (1). Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Caveolins 1 and 2 are similar in most respects, however, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell (2). Caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins (1).

Alternate Names

CAV2, Caveolin2

Gene Symbol

CAV2

Additional Caveolin-2 Products

Product Documents for Caveolin-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caveolin-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for Caveolin-2 Antibody - BSA Free

Customer Reviews for Caveolin-2 Antibody - BSA Free (1)

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  • Caveolin-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: abecular meshwork cells
    Species: Pig
    Verified Customer | Posted 11/28/2016
    Detection of caveolin2 in cultured pig trabecular meshwork cells using polyclonal Caveolin-2 Antibody (NBP1-31116) at 1:500 dilution overnight and donkey anti-rabbit Alexa 488 (1:1000) for 1 hour.
    Caveolin-2 Antibody - BSA Free NBP1-31116

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