CBL Antibody (3B12)

Novus Biologicals | Catalog # NBP2-37574

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B12
View all CBL Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CBL expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBL Antibody (3B12)

Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunocytochemistry/ Immunofluorescence: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunocytochemistry/Immunofluorescence: CBL Antibody (3B12) [NBP2-37574] - Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) [NBP2-37574] - Analysis of cancer tissues using C-CBL mouse mAb with DAB staining.
Flow Cytometry: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Flow Cytometry: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Flow Cytometry: CBL Antibody (3B12) [NBP2-37574] - Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).
Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12)BSA Free [NBP2-37574]

Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)
Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) - BSA Free [NBP2-37574]

Immunohistochemistry-Paraffin: CBL Antibody (3B12) [NBP2-37574] - Analysis of ovarian cancer tissues using C-CBL mouse mAb with DAB staining.
ELISA: CBL Antibody (3B12) - BSA Free [NBP2-37574]

ELISA: CBL Antibody (3B12) - BSA Free [NBP2-37574]

ELISA: CBL Antibody (3B12) [NBP2-37574] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CBL Antibody (3B12)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 4 using NBP2-37574 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBL

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL Antibody (3B12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CBL Antibody (3B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Customer Reviews for CBL Antibody (3B12) (1)

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Customer Images

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  • CBL Antibody (3B12)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: neonatal cardiac myocytes
    Species: Mouse
    Verified Customer | Posted 12/11/2019
    CBL
    CBL Antibody (3B12) NBP2-37574

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Protocols

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail