CBX3 Antibody

Novus Biologicals | Catalog # NB100-2418

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CBX3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EAFLNSQKAGKEKD corresponding to internal region according to NP_057671.2.

Specificity

No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CBX3 Antibody

Western Blot: CBX3 Antibody [NB100-2418]

Western Blot: CBX3 Antibody [NB100-2418]

Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NB100-2418]

Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NB100-2418]

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418]

Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418]

Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: CBX3 Antibody [NB100-2418]

Flow Cytometry: CBX3 Antibody [NB100-2418]

Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: CBX3 Antibody [NB100-2418]

Western Blot: CBX3 Antibody [NB100-2418]

Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NB100-2418]

Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NB100-2418]

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for CBX3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 - 3 ug/mL

Immunohistochemistry-Paraffin

2 - 3 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBX3

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Entrez Gene IDs

11335 (Human); 12417 (Mouse); 297093 (Rat)

Gene Symbol

CBX3

UniProt

NP_009207.2 (Human)

Additional CBX3 Products

Product Documents for CBX3 Antibody

Certificate of Analysis

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Product Specific Notices for CBX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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