CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

Novus Biologicals | Catalog # NB120-10394

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 53405.111

Format

Azide and BSA Free
View all CCL5/RANTES Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length protein (Mouse) expressed in E.coli.

Localization

Secreted

Specificity

RANTES (53405.111)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

8-25 ug/ml

Western Blot

1:100-1:2000
Application Notes
ELISA: Use at a concentration of 0.5 - 2 ug/ml. The detection limit for mouse RANTES is approximately 50.From ELISA, this antibody shows ~50% cross-reactivity with recombinant human RANTES. Use in ICC/IF was reported in scientific literature (PMID: 31760088). Neutralization : Monoclonal Anti-RANTES is measured by its ability to neutralize the biological activity of recombinant mouse RANTES. This antibody will also neutralize the bioactivity of recombinant human RANTES when used at 100x higher IgG concentration.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 5% Trehalose pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C as supplied. After reconstitution, store at 2C to 8C for 1 month and at -20 for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL5/RANTES

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse)

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

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Protocols

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FAQs

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