CCNK Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-06519
![Western Blot: CCNK Antibody [NBP1-06519]](https://beta-resources.rndsystems.com/images/products/CCNK-Antibody-Western-Blot-NBP1-06519-img0016.jpg "Western Blot: CCNK Antibody [NBP1-06519]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human cyclin K using the numbering given in entry NP_001092872.1 (GeneID 8812).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CCNK Antibody - BSA Free (NBP1-06519) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-CCNK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CCNK Antibody - BSA Free
Western Blot: CCNK Antibody [NBP1-06519]
Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519]](https://beta-resources.rndsystems.com/images/products/CCNK-Antibody-Immunohistochemistry-Paraffin-NBP1-06519-img0017.jpg "Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519]")
Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519]
Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB![Western Blot: CCNK Antibody [NBP1-06519]](https://beta-resources.rndsystems.com/images/products/CCNK-Antibody-Western-Blot-NBP1-06519-img0015.jpg "Western Blot: CCNK Antibody [NBP1-06519]")
Western Blot: CCNK Antibody [NBP1-06519]
Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Immunoprecipitation: CCNK Antibody [NBP1-06519]](https://beta-resources.rndsystems.com/images/products/CCNK-Antibody-Immunoprecipitation-NBP1-06519-img0014.jpg "Immunoprecipitation: CCNK Antibody [NBP1-06519]")
Immunoprecipitation: CCNK Antibody [NBP1-06519]
Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Applications for CCNK Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2 -10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cyclin K/CCNK
Long Name
Cyclin K
Alternate Names
CCNK, CPR4, IDDHDF
Entrez Gene IDs
8812 (Human)
Gene Symbol
CCNK
UniProt
Additional Cyclin K/CCNK Products
Product Documents for CCNK Antibody - BSA Free
Product Specific Notices for CCNK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CCNK Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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