CCR9 Antibody (4G2) - BSA Free

Novus Biologicals | Catalog # NBP3-26685

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4G2 expressed in HEK293

Format

BSA Free
View all CCR9 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human CCR9 [UniProt P51686]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR9 Antibody (4G2) - BSA Free

CCR9 Antibody (4G2)

Immunoprecipitation: CCR9 Antibody (4G2) [NBP3-26685] -

Immunoprecipitation: CCR9 Antibody (4G2) [NBP3-26685] - Immunoprecipitating CCR9 in HL-60 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26685 in HL-60 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26685 (3ug) + HL-60 whole cell lysate (500ug).
Lane 3: HL-60 whole cell lysate (20ug).
CCR9 Antibody (4G2)

Western Blot: CCR9 Antibody (4G2) [NBP3-26685] -

Western Blot: CCR9 Antibody (4G2) [NBP3-26685] - Positive Western Blot detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue.
All lanes: CCR9 Antibody at 1ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 43, 41 KDa
Observed band size: 43 KDa
CCR9 Antibody (4G2)

Immunocytochemistry/Immunofluorescence: CCR9 Antibody (4G2) [NBP3-26685] -

Immunocytochemistry/Immunofluorescence: CCR9 Antibody (4G2) [NBP3-26685] - Staining of HepG2 cells with CCR9 Antibody (4G2) at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for CCR9 Antibody (4G2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CCR9

CCR9 is encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.

Alternate Names

CCR9, CD199, CDw199

Gene Symbol

CCR9

Additional CCR9 Products

Product Documents for CCR9 Antibody (4G2) - BSA Free

Certificate of Analysis

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Product Specific Notices for CCR9 Antibody (4G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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