CCT5 Antibody (639) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43680

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Hamster

Cited:

Human, Mouse

Predicted:

Bovine (95%), Chimpanzee (100%), Porcine (96%), Rat (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 639

Format

Azide and BSA Free
View all CCT5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CCT5. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31964905). Zebrafish (83%), Xenopus laevis (86%), Chicken (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCT5 Antibody (639) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CCT5 Antibody (639) [NBP2-43680]

Immunocytochemistry/ Immunofluorescence: CCT5 Antibody (639) [NBP2-43680]

Immunocytochemistry/Immunofluorescence: CCT5 Antibody (639) [NBP2-43680] - CCT5 Antibody (639) detects CCT5 protein at centrosome and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CCT5 stained by CCT5 Antibody (639) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
CCT5 Antibody (639) - Azide and BSA Free

Western Blot: CCT5 Antibody (639) [NBP2-43680] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (NBP2-43680) diluted at 1:1000.
CCT5 Antibody (639) - Azide and BSA Free

Western Blot: CCT5 Antibody (639) [NBP2-43680] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (NBP2-43680) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for CCT5 Antibody (639) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CCT5

CCT5 encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Alternate Names

chaperonin containing TCP1, subunit 5 (epsilon), epsilon subunit

Gene Symbol

CCT5

Additional CCT5 Products

Product Documents for CCT5 Antibody (639) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CCT5 Antibody (639) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCT5 Antibody (639) - Azide and BSA Free

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Protocols

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