CCT6B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92177

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human CCT6B (NP_006575.2). AEGFEAAKIKALEVLEEVKVTKEMKRKILLDVARTSLQTKVHAELADVLTEVVVDSVLAVRRPGYPIDLFMVEIMEMKHKLGTDTKLIQGLVLDHGARHPDMKKRVEDAFILICNVSLEYEKTEVNSGFFYKTAEEKEKLVKAERKFIEDRVQKIIDLKDKVCAQSNKGFVVINQKGIDPFSLDSLAKHGIVALRRAKRRNMERLSLACGGMAVNSFEDLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCT6B Antibody - Azide and BSA Free

Western Blot: CCT6B AntibodyAzide and BSA Free [NBP2-92177]

Western Blot: CCT6B AntibodyAzide and BSA Free [NBP2-92177]

Western Blot: CCT6B Antibody [NBP2-92177] - Analysis of extracts of various cell lines, using CCT6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.
Immunocytochemistry/ Immunofluorescence: CCT6B Antibody - Azide and BSA Free [NBP2-92177]

Immunocytochemistry/ Immunofluorescence: CCT6B Antibody - Azide and BSA Free [NBP2-92177]

Immunocytochemistry/Immunofluorescence: CCT6B Antibody [NBP2-92177] - Analysis of L929 cells using CCT6B at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: CCT6B Antibody - Azide and BSA Free [NBP2-92177]

Immunocytochemistry/ Immunofluorescence: CCT6B Antibody - Azide and BSA Free [NBP2-92177]

Immunocytochemistry/Immunofluorescence: CCT6B Antibody [NBP2-92177] - Analysis of HeLa cells using CCT6B at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for CCT6B Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCT6B

CCT6B encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Alternate Names

Cctz2, CCTZ-2, CCT-zeta-2, CCT-zeta-like, chaperonin containing TCP1, subunit 6B (zeta 2), T-complex protein 1 subunit zeta-2, T-complex protein 1, zeta-2 subunit, TCP-1-zeta-2, TCP-1-zeta-like, Testis-specific protein TSA303, Testis-specific Tcp20, TSA303

Gene Symbol

CCT6B

Additional CCT6B Products

Product Documents for CCT6B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCT6B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for CCT6B Antibody - Azide and BSA Free

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Protocols

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