CD11c Antibody (N418) - Chimeric - Azide and BSA Free

Name: CD11c Antibody (N418) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-81066
Price: 239 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-81066

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # N418

Format

Azide and BSA Free

View all CD11c Primary Antibodies »

Product Specifications

Immunogen

Hamster spleen cells that had been primed to mouse spleen dendritic cells

Specificity

The antibody binds to mouse and human CD11c.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD11c Antibody (N418) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD11c Antibody (N418) - Chimeric - Azide and BSA Free [NBP2-81066]

Immunocytochemistry/Immunofluorescence: CD11c Antibody (N418) [NBP2-81066] - Immunofluorescence staining of fixed U937 cells with anti-CD11c antibody N418 [NBP2-81066]. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of N418 [NBP2-81066] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81066, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for CD11c Antibody (N418) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory responses.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD11c

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

Additional CD11c Products

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Certificate of Analysis

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Product Specific Notices for CD11c Antibody (N418) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.