CD133 Antibody (W6B3C1) - BSA Free

Novus Biologicals | Catalog # NBP3-09159

Clone W6B3C1 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # W6B3C1

Format

BSA Free
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Product Specifications

Immunogen

WERI-RB-1 retinoblastoma cell line

Specificity

The mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD133 Antibody (W6B3C1) - BSA Free

Flow Cytometry: CD133 Antibody (W6B3C1) - BSA Free [NBP3-09159]

Flow Cytometry: CD133 Antibody (W6B3C1) - BSA Free [NBP3-09159]

Flow Cytometry: CD133 Antibody (W6B3C1) [NBP3-09159] - Analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (293C3) purified, GAM-FITC.

Applications for CD133 Antibody (W6B3C1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (W6B3C1) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD133 Antibody (W6B3C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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