CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free

Name: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-52660
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52660

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Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

Block/Neutralize, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # Daclizumab

Format

Azide and BSA Free

View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

Cultured T-cells derived from a patient with Mycosis fungoides.

Specificity

Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free [NBP2-52660]

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Daclizumab (NBP2-52660, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free [NBP2-52660]

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - Cynomolgus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free [NBP2-52660]

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Rhesus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.

Applications

Application

Recommended Usage

Flow Cytometry

1:10 - 1:1000

Application Notes

This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA, biosimilar antibody, biosimilars

Gene Symbol

IL2RA

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Product Documents

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Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.