CD40 Ligand/TNFSF5 Antibody (JM11-34)

Novus Biologicals | Catalog # NBP2-66756

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM11-34 expressed in HEK293
View all CD40 Ligand/TNFSF5 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD40 Ligand/TNFSF5 aa 103-150 / 261. (SwissProt: P29965 Human; SwissProt: P27548 Mouse; SwissProt: Q9Z2V2 Rat)

Localization

Cell membrane, Cell surface, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD40 Ligand/TNFSF5 Antibody (JM11-34)

Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate
Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.
Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756]

Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for CD40 Ligand/TNFSF5 Antibody (JM11-34)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody (JM11-34)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD40 Ligand/TNFSF5 Antibody (JM11-34)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD40 Ligand/TNFSF5 Antibody (JM11-34)

There are currently no reviews for this product. Be the first to review CD40 Ligand/TNFSF5 Antibody (JM11-34) and earn rewards!

Have you used CD40 Ligand/TNFSF5 Antibody (JM11-34)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40 Ligand/TNFSF5 Antibody (JM11-34)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?

    A:

    Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies