CD40 Ligand/TNFSF5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91770

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD40 Ligand/TNFSF5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CD40 Ligand/TNFSF5 Antibody - BSA Free (NBP1-91770) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD40 Ligand/TNFSF5 Antibody - BSA Free

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human rectum shows strong cytoplasmic positivity in leukocytes.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human lymph node shows strong cytoplasmic positivity in leukocytes.
Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
CD40 Ligand/TNFSF5 Antibody - BSA Free Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody - BSA Free [NBP1-91770]

Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody - BSA Free [NBP1-91770]

Analysis in human lymph node and cerebral cortex tissues using NBP1-91770 antibody. Corresponding CD40LG RNA-seq data are presented for the same tissues.
CD40 Ligand/TNFSF5 Antibody - BSA Free Western Blot: CD40 Ligand/TNFSF5 Antibody - BSA Free [NBP1-91770]

Western Blot: CD40 Ligand/TNFSF5 Antibody - BSA Free [NBP1-91770]

Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for CD40 Ligand/TNFSF5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD40 Ligand/TNFSF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40 Ligand/TNFSF5 Antibody - BSA Free

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    • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
      A: Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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